ELASTASE RELEASE FROM GINGIVAL CREVICULAR AND PERIPHERAL NEUTROPHILS IN PERIODONTITIS AND HEALTH
ELASTASE RELEASE FROM GINGIVAL CREVICULAR AND PERIPHERAL NEUTROPHILS IN PERIODONTITIS AND HEALTH
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DOI：
10.1016/0003-9969(94)90002-7
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发表时间：
1994-09-01
影响因子：
3
通讯作者：
CIMASONI, G
中科院分区：
文献类型：
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作者：
GIANNOPOULOU, C;DEMEURISSE, C;CIMASONI, G
炎症期间龈沟内弹性蛋白酶的活性显着增加。研究人员对 7 名牙龈炎患者、6 名成人牙周炎患者、7 名快速进展性牙周炎患者和 9 名健康受试者研究了牙缝和外周多形核白细胞 (PMN) 释放的这种酶。从血液中分离出外周中性粒细胞，从洗龈液中分离出缝隙中的中性粒细胞。用细胞松弛素 B 预孵育后，在磷酸盐缓冲盐水（自发释放）或含有甲酰基甲硫氨酰-亮氨酰-苯基-丙氨酸（刺激释放）的相同缓冲液中孵育相同数量的裂隙细胞和外周细胞。通过荧光技术测量上清液中的弹性蛋白酶活性。结果证实，与外周中性粒细胞相比，裂隙细胞表现出更高的弹性蛋白酶自发释放和更低的刺激释放。与从牙龈炎或牙周炎患者获得的细胞相比，对照组中自发释放的或在刺激裂隙细胞后释放的弹性蛋白酶活性显着较低。从口袋较深的患者身上收集的缝隙中性粒细胞似乎比对照组释放出更多的弹性蛋白酶。
The activity of elastase increases significantly in the gingival sulcus during inflammation. The release of this enzyme from crevicular and peripheral polymorphonuclear leucocytes (PMNs) was studied in seven patients with gingivitis, six with adult periodontitis, seven with rapidly progressive periodontitis and in nine healthy subjects. Peripheral PMNs were isolated from blood and crevicular PMNs from gingival washings. After preincubation with cytochalasin B, the same numbers of crevicular and peripheral cells were incubated either in phosphate-buffered saline (spontaneous release) or in the same buffer containing formyl methionyl-leucyl-phenyl-alanine (stimulated release). Elastase activity was measured in the supernatant by a fluorimetric technique. The results confirm that compared to peripheral PMNs, crevicular cells show a higher spontaneous release of elastase and a lower stimulated release. The activity of elastase released either spontaneously or after stimulation of crevicular cells was significantly lower in the controls as compared with cells obtained from patients with gingivitis or periodontitis. Crevicular PMNs collected from patients with deeper pockets appear to release larger amounts of elastase than those from controls.