Pulmonary artery haemorrhage in newborn calves following bluetongue virus serotype 8 experimental infections of pregnant heifers
Pulmonary artery haemorrhage in newborn calves following bluetongue virus serotype 8 experimental infections of pregnant heifers
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DOI：
10.1016/j.vetmic.2013.08.016
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发表时间：
2013-12-27
影响因子：
3.3
通讯作者：
Saegerman, Claude
中科院分区：
文献类型：
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作者：
Martinelle, Ludovic;Dal Pozzo, Fabiana;Saegerman, Claude
2006年欧洲牲畜中出现的蓝舌病（BT）提出了许多问题，包括牛胎儿感染的发生和流行病学意义。为了澄清这些方面，在隔离设施（生物安全等级3）中，接种疫苗和未接种疫苗的怀孕母牛先后两次感染北欧8型蓝舌病病毒（BTV-8）。该研究在产犊2个月后终止，并对水坝及其后代进行尸检。在整个研究过程中，通过临床评分和循环中和抗体的存在，以及产犊后血液和牛奶中感染性病毒和病毒RNA的存在，对牛进行了监测。在妊娠120天感染的4头小牛，其中1头来自接种疫苗的母鹿，3头来自未接种疫苗的母鹿，尸检时发现有明显的肺动脉出血。虽然肺动脉出血是BT的高度特征，但在这些小牛中未检测到病毒RNA。此外，虽然妊娠前感染的母牛所生的小牛没有出现致畸性BTV典型脑损伤，但一些出生时的病变提示子宫内BTV感染。尽管缺乏病毒RNA检测，但在以牛BTV胎盘杂交为主要主题的多重调查背景下，肺动脉出血的存在值得作为一个新的观察报告。(C) 2013 Elsevier B.V.版权所有
The emergence of bluetongue disease (BT) among livestock in Europe in 2006 raised many questions including the occurrence and epidemiological significance of foetal infections in cattle. To clarify these aspects, vaccinated and unvaccinated pregnant heifers were sequentially infected twice in an isolation facility (biosafety level 3) with a northern European outbreak strain of Bluetongue virus serotype 8 (BTV-8). The study was terminated 2 months after calving with necropsy of the dams and their offspring. The cattle were monitored throughout the study by clinical scoring and for the presence of circulating neutralising antibodies, and after calving for the presence of infectious virus and viral RNA in blood and milk. Four calves, one born from a vaccinated dam and three from non-vaccinated ones, that were infected at 120 days of gestation had obvious haemorrhage of the pulmonary artery at necropsy. Although haemorrhage of the pulmonary artery is highly characteristic of BT, viral RNA was not detected in any of these calves. Furthermore, although none of the calves born from heifers infected prior to midgestation had teratogenic BTV typical brain lesions, some had lesions at birth suggestive of in utero BTV infection. Despite the lack of viral RNA detection, the presence of haemorrhage of the pulmonary artery deserves to be reported as a new observation in the context of the multiple investigations having as main subject the BTV placental crossing in cattle. (C) 2013 Elsevier B.V. All rights reserved.