Age-associated changes in protein oxidation and proteasome activities in rat brain: Modulation by antioxidants
Age-associated changes in protein oxidation and proteasome activities in rat brain: Modulation by antioxidants
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DOI：
10.1016/j.bbrc.2005.07.201
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发表时间：
2005-10-21
影响因子：
3.1
通讯作者：
Jenner, P
中科院分区：
文献类型：
--
作者：
El Mohsen, MMA;Iravani, MM;Jenner, P
衰老的自由基理论假设，与年龄相关的神经退行性变是由促氧化剂和抗氧化剂之间的不平衡引起的，从而导致氧化应激。目前的研究表明，与对照组（3-5个月）相比，15-16个月大的雄性大鼠的脂褐素沉积和蛋白质羰基水平增加，表明大脑对年龄相关氧化应激的敏感性存在区域差异。海马是最容易受到影响的区域相比，皮层和小脑。然而，蛋白酶体的酶活性并没有受到年龄的影响，在任何大脑区域的研究。用褪黑激素或辅酶Q10治疗4周可降低海马的脂褐素含量和羰基水平。然而，褪黑激素和辅酶Q10治疗抑制β-谷氨酰肽水解酶活性。这表明这些分子可以独立于其抗氧化作用改变蛋白酶体功能。(c)2005年爱思唯尔公司All rights reserved.
The free radical theory of ageing postulates that age-associated neurodegeneration is caused by an imbalance between pro-oxidants and antioxidants resulting in oxidative stress. The current study showed regional variation in brain susceptibility to age-associated oxidative stress as shown by increased lipofuscin deposition and protein carbonyl levels in male rats of age 15-16 months compared to control ones (3-5 months). The hippocampus is the area most vulnerable to change compared to the cortex and cerebellum. However, proteasomal enzyme activity was not affected by age in any of the brain regions studied. Treatment with melatonin or coenzyme Q10 for 4 weeks reduced the lipofuscin content of the hippocampus and carbonyl level. However, both melatonin and coenzyme Q10 treatments inhibited beta-glutamyl peptide hydrolase activity. This suggests that these molecules can alter proteasome function independently of their antioxidant actions. (c) 2005 Elsevier Inc. All rights reserved.