Modified Oxygen Radical Absorbance Capacity Assay that can be Implemented at Low Temperatures: A pilot study
Modified Oxygen Radical Absorbance Capacity Assay that can be Implemented at Low Temperatures: A pilot study
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DOI：
10.3136/fstr.19.269
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发表时间：
2013-03-01
影响因子：
0.6
通讯作者：
Fujii, Hirotada
中科院分区：
文献类型：
--
作者：
Kohri, Shunji;Fujii, Hirotada
本文对一种改进的基于荧光的氧自由基吸收能力测定方法(ORAC-FL)的可行性进行了评价。在改进的ORAC-FL法中，2，2‘-偶氮二(2-氨基丙烷)二盐酸盐(AAPH)在紫外光照射下，在短时间内产生大量的自由基。重要的是，不需要加热来产生自由基，同时由于荧光快速衰减，缩短了监测荧光强度(FI)所需的时间。本研究确定了改良测定的最佳实验条件，并用改进的测定方法评价了两个样品在室温下的抗氧化能力。虽然获得了相对ORAC值，但改进后的检测方法的某些方面需要进一步优化，才能最终用于实际应用。
In this paper, the feasibility of a modified oxygen radical absorbance capacity assay based on fluorescence (ORAC-FL) was evaluated. In the modified ORAC-FL assay, a large quantity of radicals is generated in a short period of time by photolysis of 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), through illumination with ultraviolet light. Importantly, no heating is required to generate the radicals, while the time required to monitor the fluorescence intensity (FI) is shortened, owing to the rapid fluorescence decay. In this study, the optimal experimental conditions for the modified assay were determined and the antioxidant capacity of two samples was evaluated at room temperature using the modified assay. Although relative ORAC values were obtained, some aspects of the modified assay require further optimization for eventual practical use.