Differential regulation of pro-inflammatory cytokines during wound healing in normal and glucocorticoid-treated mice
Differential regulation of pro-inflammatory cytokines during wound healing in normal and glucocorticoid-treated mice
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DOI：
10.1006/cyto.1996.0074
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发表时间：
1996-07-01
期刊：
影响因子：
3.8
通讯作者：
Werner, S
中科院分区：
文献类型：
--
作者：
Hubner, G;Brauchle, M;Werner, S
促炎细胞因子在创伤修复过程中起重要作用，然而，这些细胞因子在正常和受损创伤愈合过程中的时间和空间表达模式知之甚少。（IL-α和β）和肿瘤坏死因子α这些细胞因子的最高水平早在创伤后12-24小时就观察到，在伤口愈合的增殖期完成后，这些细胞因子的mRNA水平恢复到基础水平。在伤口修复的早期阶段，促炎细胞因子主要在多形核白细胞中表达，表明这些细胞在伤口愈合的起始中具有新的功能。在修复过程的后期阶段，在巨噬细胞中也观察到IL-1 α、IL-1 β和TNF-α的表达。在伤口边缘的过度增殖上皮中检测到TNF-α，在毛囊的角质形成细胞中发现IL-1 α，在愈合受损的糖皮质激素治疗的小鼠中，损伤后这些细胞因子的诱导在伤口修复期间显著减少。这一发现表明伤口愈合缺陷与受损的细胞因子表达相关，并表明这些基因的早期诱导对正常修复很重要。(C)1996年学术出版社
It has long been speculated that pro-inflammatory cytokines play an important role in wound repair, However, little is known about the temporal and spatial expression pattern of these cytokines during normal and impaired wound healing, In this study we show a strong and early induction of interleukins 1 alpha and beta (IL-alpha and beta) and of tumour necrosis factor alpha (TNF-alpha) expression after cutaneous injury, Highest levels of these cytokines were seen as early as 12-24 h after wounding, After completion of the proliferative phase of wound healing, mRNA levels of these cytokines returned to the basal level. During the early phase of wound repair, proinflammatory cytokines were predominantly expressed in polymorphonuclear leukocytes, suggesting a novel function of these cells in the initiation of wound healing, At later stages of the repair process, expression of IL-1 alpha, IL-1 beta and TNF-alpha was also seen in macrophages, Furthermore, TNF-alpha was detected in the hyperproliferative epithelium at the wound edge and IL-1 alpha was found in keratinocytes of the hair follicles, Induction of these cytokines after injury was significantly reduced during wound repair in healing-impaired glucocorticoid-treated mice. This finding demonstrates that wound healing defects are associated with impaired cytokine expression and suggests that the early induction of these genes is important for normal repair. (C) 1996 Academic Press Limited