IMPROVEMENT OF THE DIDEOXY CHAIN TERMINATION METHOD OF DNA SEQUENCING BY USE OF DEOXY-7-DEAZAGUANOSINE TRIPHOSPHATE IN PLACE OF DGTP
IMPROVEMENT OF THE DIDEOXY CHAIN TERMINATION METHOD OF DNA SEQUENCING BY USE OF DEOXY-7-DEAZAGUANOSINE TRIPHOSPHATE IN PLACE OF DGTP
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DOI：
10.1093/nar/14.3.1319
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发表时间：
1986-02-11
影响因子：
14.9
通讯作者：
SEELA, F
中科院分区：
文献类型：
--
作者：
MIZUSAWA, S;NISHIMURA, S;SEELA, F
用脱氧-7-二氮杂鸟苷三磷酸（dc7GTP）代替dGTP的二脱氧链终止法是非常有用的。由于条带压缩，使用dGTP的原始方法无法测序具有85% GC含量的人类N-mycgene的一部分。然而，这部分的核苷酸序列是通过修改后的方法对两条链的分析明确确定的。利用dc7GTP改进了DNA测序的双脱氧链终止法。
The dideoxy chain termination method using deoxy-7-deaza-guanosine triphosphate (dc7GTP)in place of dGTP was found to be very useful. Sequencing of a part of the human N-mycgene having 85% GC content is impossible by the original method using dGTP, because of compression of bands. However, the nucleotide sequence of this part was unambiguously determined by analysis of both strands by the modified method. Use of dc7GTP is concluded to improve the dideoxy chain termination method for DNA sequencing.