Anchoring protein crystals to mounting loops with hydrogel using inkjet technology
Anchoring protein crystals to mounting loops with hydrogel using inkjet technology
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使用喷墨技术将蛋白质晶体锚定到水凝胶安装环上
DOI：
10.1107/s139900471401476x
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Min Yao and Isao Tanaka
中科院分区：
文献类型：
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作者：
Akira Shinoda;Yoshikazu Tanaka;Min Yao and Isao Tanaka
X射线晶体学是基于结构的药物发现的重要技术，主要是因为它是唯一可以揭示配体是否与靶蛋白结合以及结合位置和方式的技术。然而，通过X射线晶体学进行的配体筛选涉及晶体浸泡实验，该实验通常手动进行。因此，对于筛选大量的候选配体，通量不令人满意。在这项研究中，锚蛋白质晶体安装环通过使用凝胶和喷墨技术已开发的技术，该方法允许浸泡的安装晶体在含配体的解决方案。这项新技术可能有助于设计全自动药物筛选管道。
X-ray crystallography is an important technique for structure-based drug discovery, mainly because it is the only technique that can reveal whether a ligand binds to the target protein as well as where and how it binds. However, ligand screening by X-ray crystallography involves a crystal-soaking experiment, which is usually performed manually. Thus, the throughput is not satisfactory for screening large numbers of candidate ligands. In this study, a technique to anchor protein crystals to mounting loops by using gel and inkjet technology has been developed; the method allows soaking of the mounted crystals in ligand-containing solution. This new technique may assist in the design of a fully automated drug-screening pipeline.