The sulphorhodamine (SRB) assay and other approaches to testing plant extracts and derived compounds for activities related to reputed anticancer activity
The sulphorhodamine (SRB) assay and other approaches to testing plant extracts and derived compounds for activities related to reputed anticancer activity
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DOI：
10.1016/j.ymeth.2007.01.003
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发表时间：
2007-08-01
期刊：
影响因子：
4.8
通讯作者：
Lee, C. C.
中科院分区：
文献类型：
--
作者：
Houghton, Peter;Fang, Rui;Lee, C. C.
由于在过去的50年中，寻找潜在的抗癌药物的主要方法是基于对哺乳动物癌细胞系的选择性细胞毒性作用，基于细胞的细胞毒性方法进行了描述和比较。详细描述了磺基罗丹明B（SR B）测定法作为首选方法，并且还开发了一种新方法，该方法基于以下假设：在某些情况下，天然存在的化合物本身作为前药而不是活性化合物。因此，提取物或化合物在测试之前与模拟体内代谢过程的系统一起预孵育。该方法已使用已知化合物进行了验证，鸢尾提取物在用β-葡萄糖苷酶处理后显示出更强的细胞毒性。此外，还讨论了基于哺乳动物细胞的生物测定，包括抗氧化剂和上调某些细胞自卫机制，这些机制与癌症的预防和治疗有关。高良姜提取物诱导谷胱甘肽-S-转铁酶（GST）的活性在培养的肝细胞，这是追溯到苯丙素类，特别是V-乙酰氧基chavicol乙酸酯。(C)2007年爱思唯尔公司All rights reserved.
Since the major approach in searching for potential anticancer agents over the last 50 years has been based on selective cytotoxic effects on mammalian cancer cell lines, cell-based methods for cytotoxicity are described and compared. The sulphorhodamine B (SRB) assay is described in detail as the preferred method and also a novel approach has been developed which is based on the hypothesis that, in some circumstances, the naturally occurring compounds act as prodrugs rather than active compounds in their own right. Consequently, extracts or compounds are pre-incubated with systems modelling metabolic processes in the body before being tested. The methods have been validated using known compounds and Iris tectorum extracts have been shown to be more cytotoxic after treatment with beta-glucosidase. In addition bioassays based on mammalian cells involving antioxidant and upregulation of some cellular self-defence mechanisms are discussed which are related to prevention as well as treatment of cancer. Extracts of Alpinia officinarum induced glutathione-S-trans fe rase (GST) activity in cultured hepatocytes and this was traced to the phenylpropanoids present, especially V-acetoxychavicol acetate. (C) 2007 Elsevier Inc. All rights reserved.