A new microanalytical method for determination of hydroxyproline in connective tissues.
A new microanalytical method for determination of hydroxyproline in connective tissues.
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一种测定结缔组织中羟脯氨酸的新微量分析方法。
DOI：
10.2302/kjm.27.43
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
S. Saito
中科院分区：
文献类型：
--
作者：
S. Inayama;T. Shibata;Junichi Ohtsuki;S. Saito
在普罗科普常规方法的基础上，建立了测定微量结缔组织(0.05-5 mg)中羟脯氨酸的微量分析方法。新方法简单、快速、灵敏、线性范围较宽(0.04-20μg)，即使专家或新手使用也具有很高的重现性。本文主要介绍了该方法(规程)的标准分析程序，该程序是对常规方法进行必要的修改而产生的。
A microanalytical method for the determination of hydroxyproline in a microquantity of connective tissues (0.05-5mg) has been developed on the basis of Prockop's conventional methods. The new method is simple, rapid, sensitive, and linear in a wider assay range (0.04-20μg), with a high reproducibility even when used by experts or novices. The present communication mainly describes the standard assay procedure of the method (protocol), which has been brought about by essential modification of the conventional method.