Identification of a Vibrio cholerae ToxR-activated gene (tagD) that is physically linked to the toxin-coregulated pilus (tcp) gene cluster.
Identification of a Vibrio cholerae ToxR-activated gene (tagD) that is physically linked to the toxin-coregulated pilus (tcp) gene cluster.
复制标题
霍乱弧菌 ToxR 激活基因 (tagD) 的鉴定，该基因与毒素共调节菌毛 (tcp) 基因簇物理相连。
DOI：
10.1016/0378-1119(94)90240-2
复制
发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
Peterson,KM
中科院分区：
文献类型：
--
作者：
Hughes,KJ;Everiss,KD;Harkey,CW;Peterson,KM
毒素协同调节菌毛（TCP）编码基因簇（TCP）指定IV型菌毛是霍乱弧菌的主要定植决定因素。我们已经确定了一个基因200 bp上游的TCP簇，需要ToxR的表达。我们已经将该基因命名为tagD（T oxR-a激活基因），并且已经表明tagD在600-nt转录物上编码。推断的tagD产物是164个氨基酸的多肽（20 kDa）。有趣的是，TagD与被认为在菌毛合成或组装中起作用的血链球菌12的蛋白质具有高度相似性。tagD和Ss 12蛋白之间的高度相似性提供了初步证据，表明tagD代表了tcp簇的另一个成员。
The toxin-coregulated pilus (TCP)-encoding gene cluster (tcp) specifies a type-IV pilus that is a major colonization determinant of Vibrio cholerae. We have identified a gene 200 bp upstream from the tcp cluster that requires ToxR for expression. We have designated this gene tagD (T oxR-a ctivated g ene) and have shown that tagD is encoded on a 600-nt transcript. The deduced tagD product is a 164-amino-acid polypeptide (20 kDa). Interestingly, TagD shares a high degree of similarity to a protein of Streptococcus sanguis 12 that is thought to play a role in fimbriae synthesis or assembly. The high degree of similarity between tagD and the Ss 12 protein provides preliminary evidence that tagD represents an additional member of the tcp cluster.