Natural Carbon Isotope Abundance of Plasma Metabolites and Liver Tissue Differs between Diabetic and Non-Diabetic Zucker Diabetic Fatty Rats
Natural Carbon Isotope Abundance of Plasma Metabolites and Liver Tissue Differs between Diabetic and Non-Diabetic Zucker Diabetic Fatty Rats
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DOI：
10.1371/journal.pone.0074866
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发表时间：
2013-09-23
期刊：
影响因子：
3.7
通讯作者：
Kochhar, Sunil
中科院分区：
文献类型：
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作者：
Godin, Jean-Philippe;Ross, Alastair B.;Kochhar, Sunil
背景：说到碳同位素丰度，“你吃什么，你就是什么”是对人类和动物的准确概括。在生物材料中，天然的(13)C/C-12比率由于饮食组成(主要是由于摄入C-3和C-4代谢植物)以及生化反应(同位素效应和同位素分馏)中的“轻”和“重”同位素的区分而受到微小变化的影响。方法/主要发现：碳同位素丰度是在喂养相同饮食的ZDF(FA/+)和ZDF(FA/FA)中测量的(分别是瘦的和肥胖的糖尿病大鼠)。通过高精度同位素比值质谱仪分析血浆代谢物(葡萄糖和非酯化脂肪酸)、呼吸和肝组织，首次证明了根据血糖、棕榈酸、油酸、亚油酸、花生四烯酸和肝组织体积分析，ZDF(FA/+)和ZDF(FA/FA)大鼠之间的代谢碳同位素丰度是可以统计区分的(P
Background: 'You are what you eat' is an accurate summary for humans and animals when it comes to carbon isotope abundance. In biological material, natural(13)C/C-12 ratio is subject to minute variations due to diet composition (mainly from ingestion of C-3 and C-4 metabolism plants) and to the discrimination between 'light' and 'heavy' isotopes during biochemical reactions (isotope effects and isotopic fractionation).Methodology/Principal Findings: Carbon isotopic abundance was measured in ZDF (fa/+) and ZDF (fa/fa), (lean and obese-diabetic rats respectively) fed the same diet. By analysing plasma metabolites (glucose and non-esterified fatty acids), breath and liver tissue by high-precision isotope ratio mass spectrometry, we demonstrate for the first time statistically distinguishable metabolic carbon isotope abundance between ZDF (fa/+) and ZDF (fa/fa) rats based on plasma glucose, palmitic, oleic, linoleic, arachidonic acids and bulk analysis of liver tissue (P