Serological Response in RT-PCR Confirmed H1N1-2009 Influenza A by Hemagglutination Inhibition and Virus Neutralization Assays: An Observational Study
Serological Response in RT-PCR Confirmed H1N1-2009 Influenza A by Hemagglutination Inhibition and Virus Neutralization Assays: An Observational Study
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DOI：
10.1371/journal.pone.0012474
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发表时间：
2010-08-30
期刊：
影响因子：
3.7
通讯作者：
Leo, Yee-Sin
中科院分区：
文献类型：
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作者：
Chen, Mark I.;Barr, Ian G.;Leo, Yee-Sin
背景：我们描述了通过逆转录酶聚合酶链式反应 (RT-PCR) 证实的 H1N1-2009 甲型流感感染后的血清学反应。方法和主要发现：该研究包括入院患者、血清流行病学队列研究的受试者以及从新加坡爆发研究中确定的参与者。通过血凝抑制 (HI) 和病毒微量中和 (VM) 测定，分析基线（第一个可用血液样本）和后续血液样本中 H1N1-2009 和最近流行的季节性甲型流感病毒株的抗体滴度。对 118 例 H1N1-2009 病例的 267 个样本进行了分析。 HI 的几何平均滴度在第 30 至 39 天之间达到峰值 123（95% 置信区间，CI 43-356）。当将分析限制为 45 名参与者（发病后 5 天内收集基线血清，发病后 15 天或更长时间收集随访血清）时，观察到血清转化（抗体增加四倍或更多）的机会最大化；对于这些参与者，82% 和 89% 的人分别通过 HI 和 VM 血清转化为 A/California/7/2009 H1N1。季节性 A/Brisbane/59/2007 H1N1、A/Brisbane/10/2007 H3N2 和 A/Wisconsin/15/2009 H3N2 的交叉反应抗体滴度分别在 20%、18% 和 16% 的参与者中增加四倍或以上。 结论和意义：适当定时的配对血清学检测80-90% RT-PCR 确诊 H1N1-2009； H1N1-2009 感染产生的抗体与季节性流感病毒发生交叉反应。
Background: We describe the serological response following H1N1-2009 influenza A infections confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR).Methodology and Principal Findings: The study included patients admitted to hospital, subjects of a seroepidemiologic cohort study, and participants identified from outbreak studies in Singapore. Baseline (first available blood sample) and follow-up blood samples were analyzed for antibody titers to H1N1-2009 and recently circulating seasonal influenza A virus strains by hemagglutination inhibition (HI) and virus micro-neutralization (VM) assays. 267 samples from 118 cases of H1N1-2009 were analyzed. Geometric mean titers by HI peaked at 123 (95% confidence interval, CI 43-356) between days 30 to 39. The chance of observing seroconversion (four-fold or greater increase of antibodies) was maximized when restricting analysis to 45 participants with baseline sera collected within 5 days of onset and follow-up sera collected 15 or more days after onset; for these participants, 82% and 89% seroconverted to A/California/7/2009 H1N1 by HI and VM respectively. A four-fold or greater increase in cross-reactive antibody titers to seasonal A/Brisbane/59/2007 H1N1, A/Brisbane/10/2007 H3N2 and A/Wisconsin/15/2009 H3N2 occurred in 20%, 18% and 16% of participants respectively.Conclusions and Significance: Appropriately timed paired serology detects 80-90% RT-PCR confirmed H1N1-2009; Antibodies from infection with H1N1-2009 cross-reacted with seasonal influenza viruses.