Evolutionarily dynamic roles of a PUF RNA-binding protein in the somatic development of Caenorhabditis briggsae.
Evolutionarily dynamic roles of a PUF RNA-binding protein in the somatic development of Caenorhabditis briggsae.
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PUF RNA 结合蛋白在 Caenorhabditis briggsae 体细胞发育中的进化动态作用。
DOI：
10.1002/jez.b.22550
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Haag,EricS
中科院分区：
文献类型：
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作者：
Liu,Qinwen;Haag,EricS
基因复制和分化已成为发育进化的一个重要方面。隐杆线虫的基因组编码一个古老的PUF RNA结合蛋白家族。大多数与种系发育有关，并且经常与同一亚科的相似物重复。一个例外是isCbr - puf - 2（三种绿杆线虫puf - 2亚家族相似物之一），这是第二幼虫阶段发育所必需的。在这里，我们提供了cbr - puf - 2的详细功能表征。cbr - puf - 2突变动物的幼虫停滞是由细菌食物的低效分解引起的，从而导致饥饿。Cbr - puf - 2在早期幼虫阶段肌肉末梢球的正常研磨周期中是必需的，并在该组织中短暂表达。此外，对捕获幼虫的抢救表明，cbr - puf - 2也促进了正常的外阴发育。它在锚定细胞（诱导外阴命运）和外阴肌肉中表达，但在外阴前体细胞（VPCs）本身中不表达。这与秀丽隐杆线虫（aenorhabditis eleganshomologisbf‐1/2）中描述的VPC自主抑制外阴发育形成对比。这些PUF蛋白的不同作用发生在外阴和咽部高度保守的解剖结构中，表明普遍的发育系统漂移（DSD）。因为Cbr - PUF - 2与其类似物和c具有RNA结合特异性。我们认为，RNA结合蛋白的功能新颖性通过其表达位点的变化而进化，可能与目标mrna的顺式调控进化相一致。Exp。黑旋风。（Mol. Dev. evolution .）[j] .科学通报，2014。©2013 Wiley期刊公司
Gene duplication and divergence has emerged as an important aspect of developmental evolution. The genomes ofCaenorhabditisnematodes encode an ancient family of PUF RNA‐binding proteins. Most have been implicated in germline development, and are often redundant with paralogs of the same sub‐family. An exception isCbr‐puf‐2(one of threeCaenorhabditis briggsaePUF‐2 sub‐family paralogs), which is required for development past the second larval stage. Here, we provide a detailed functional characterization ofCbr‐puf‐2. The larval arrest ofCbr‐puf‐2mutant animals is caused by inefficient breakdown of bacterial food, which leads to starvation.Cbr‐puf‐2is required for the normal grinding cycle of the muscular terminal bulb during early larval stages, and is transiently expressed in this tissue. In addition, rescue of larval arrest reveals thatCbr‐puf‐2also promotes normal vulval development. It is expressed in the anchor cell (which induces vulval fate) and vulval muscles, but not in the vulva precursor cells (VPCs) themselves. This contrasts with the VPC‐autonomous repression of vulval development described for theCaenorhabditis eleganshomologsfbf‐1/2. These different roles for PUF proteins occur even as the vulva and pharynx maintain highly conserved anatomies acrossCaenorhabditis, indicating pervasive developmental system drift (DSD). Because Cbr‐PUF‐2 shares RNA‐binding specificity with its paralogs and withC. elegansFBF, we suggest that functional novelty of RNA‐binding proteins evolves through changes in the site of their expression, perhaps in concert withcis‐regulatory evolution in target mRNAs.J. Exp. Zool. (Mol. Dev. Evol.) 322B: 129–141, 2014. © 2013 Wiley Periodicals, Inc.