The interplay between toxin-releasing β-glucosidase and plant iridoid glycosides impairs larval development in a generalist caterpillar, Grammia incorrupta (Arctiidae).
The interplay between toxin-releasing β-glucosidase and plant iridoid glycosides impairs larval development in a generalist caterpillar, Grammia incorrupta (Arctiidae).
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毒素释放β-葡萄糖苷酶和植物环烯醚萜苷之间的相互作用损害多面性毛毛虫Grammia incorrupta（Arctiidae）的幼虫发育
DOI：
10.1016/j.ibmb.2012.02.004
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发表时间：
2012
影响因子：
3.8
通讯作者：
Dobler S.
中科院分区：
文献类型：
--
作者：
Pankoke H;Bowers D;Dobler S.
食草动物与杂食的饮食习惯必须科普不同的饮食质量。植物次生化学是引起寄主植物适宜性变异的重要原因之一。我们研究了如何喂养植物含有这样一组化合物，环烯醚萜苷，可能会影响生长和酶的活性在一个多食性的毛虫，在50个不同的家庭超过80种植物的饲料。幼虫的多食性arctiid，Grammia incorrupta，饲养专门的两种植物之一，其中一种含有环烯醚萜苷（车前，Plantago lanceolata，Plantinaceae），而另一个不（蒲公英，菊科）。测定了两种寄主植物上的幼虫体重，并测定了末龄幼虫中肠匀浆中β-葡萄糖苷酶的活性和动力学性质，同时使用标准底物4-硝基苯-β-d-葡萄糖（NPβGlc）和环烯醚萜苷桃叶珊瑚苷（P. lanceolata中两种主要环烯醚萜苷之一）。与在铁皮石斛上的幼虫相比，在披针叶石斛上取食的幼虫体重显著更轻，发育更慢。虽然当饲喂披针叶杉时，用NPβGlc测定的幼虫中肠β-葡萄糖苷酶活性显著降低，桃叶珊瑚苷基本上被水解，并且幼虫对两种底物的β-葡萄糖苷酶活性与幼虫体重呈负相关。我们的研究结果表明，含有高浓度环烯醚萜苷的宿主植物对这种通性昆虫植食性幼虫的发育有负面影响。这很可能是由于幼虫中肠中植物糖苷的水解导致有毒糖苷配基的释放。将幼虫体重的减少与环烯醚萜苷裂解β-葡萄糖苷酶的毒素释放作用联系起来，我们的结果因此支持解毒限制假说，表明仅以披针叶松为食的幼虫的适应性成本。因此，除了中肠β-葡萄糖苷酶活性的适应性调节外，作为行为适应的宿主植物转换可能是通才食草动物规避植物次生化合物负面影响的先决条件。
Herbivores with polyphagous feeding habits must cope with a diet that varies in quality. One of the most important sources of this variation in host plant suitability is plant secondary chemistry. We examined how feeding on plants containing one such group of compounds, the iridoid glycosides, might affect the growth and enzymatic activity in a polyphagous caterpillar that feeds on over 80 plant species in 50 different families. Larvae of the polyphagous arctiid, Grammia incorrupta, were reared exclusively on one of two plant species, one of which contains iridoid glycosides (Plantago lanceolata, Plantaginaceae) while the other does not (Taraxacum officinale, Asteraceae). Larval weight was measured on the two host plants, and midgut homogenates of last instar larvae were then assayed for activity and kinetic properties of β-glucosidases, using both a standard substrate, 4-nitrophenyl-β-d-glucose (NPβGlc), and the iridoid glycoside aucubin, one of the two main iridoid glycosides in P. lanceolata. Larvae feeding on P. lanceolata weighed significantly less and developed more slowly compared to larvae on T. officinale. While the larval midgut β-glucosidase activity determined with NPβGlc was significantly decreased when fed on P. lanceolata, aucubin was substantially hydrolyzed and the larval β-glucosidase activity towards both substrates correlated negatively with larval weight. Our results demonstrate that host plants containing high concentrations of iridoid glycosides have a negative impact on larval development of this generalist insect herbivore. This is most likely due to the hydrolysis of plant glycosides in the larval midgut which results in the release of toxic aglycones. Linking the reduced larval weight to the toxin-releasing action of an iridoid glycoside cleaving β-glucosidase, our results thus support the detoxification limitation hypothesis, suggesting fitness costs for the larvae feeding solely on P. lanceolata. Thus, in addition to the adaptive regulation of midgut β-glucosidase activity, host plant switching as a behavioral adaptation might be a prerequisite for generalist herbivores that allows them to circumvent the negative effects of plant secondary compounds.
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影响因子：
2.2
作者：
Helga Pankoke;M. Bowers;S. Dobler
通讯作者：
Helga Pankoke;M. Bowers;S. Dobler
青凤蝶亚种中肠β-葡萄糖苷酶对酚苷的水解
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
R. Lindroth
通讯作者：
R. Lindroth
几种牛螟蛾（鳞翅目：牛螟科）中吡咯里西啶生物碱的封存
影响因子：
2.3
作者：
E. V. Nickisch;Michael Wink
通讯作者：
Michael Wink
从 Zygaena trifolii Esper, 1783（昆虫纲，鳞翅目）血淋巴中纯化和鉴定 α-葡萄糖苷酶（亚麻酶）
DOI：
10.1007/bf01974565
发表时间：
1989
期刊：
Experientia
影响因子：
--
作者：
S. Franzl;I. Ackermann;A. Nahrstedt
通讯作者：
A. Nahrstedt
DOI：
10.1016/0300-9629(94)90362-x
发表时间：
1994-04-01
影响因子：
2.3
作者：
FERREIRA, C;CAPELLA, AN;TERRA, WR
通讯作者：
TERRA, WR