Differential Shh, Bmp and Wnt gene expressions during craniofacial development in mice
Differential Shh, Bmp and Wnt gene expressions during craniofacial development in mice
复制标题
DOI：
10.1016/j.acthis.2009.05.007
复制
发表时间：
2010-01-01
影响因子：
2.5
通讯作者：
Nunes, Fabio Daumas
中科院分区：
文献类型：
--
作者：
Silva Paiva, Katiucia Batista;Silva-Valenzuela, Maria das Gracas;Nunes, Fabio Daumas
本研究采用比较非同位素原位杂交技术，比较了小鼠颅面发育早期BMP-4、Wnt-5a和Shh基因的表达。在胚胎发育的各个阶段（从8.5- 13.5天的胚胎- E8.5-13.5）研究了野生型C57 BL/6 J小鼠。在早期牙形成过程中，BMP-4，Shh和Wnt-5a的成绩单共定位在牙齿起始阶段。在E8.5，Shh mRNA表达仅限于间脑和咽内胚层。在上下颌骨骨化前，间充质细胞和Meckel软骨周围有Bmp-4和Wnt-5a信号。在腭发育过程中，Shh只在上皮中表达，Wnt-5a只在腭突间充质中表达。在舌的发育过程中，Shh的表达被发现在间充质中，可能有助于舌的migenesis，而Wnt-5a的信号是在上皮中，可能在基板的发展和乳头的形成。综上所述，这些研究结果表明，BMP-4，Shh和Wnt-5a基因的表达可能共同作用于上皮间质的相互作用发生在几个方面的早期小鼠颅面发育，如牙，神经元的发育，上颌骨和下颌骨骨化，腭发育和舌的形成。(C)2009年Elsevier GmbH。All rights reserved.
In this study, Bmp-4, Wnt-5a and Shh gene expressions were compared during early craniofacial development in mice by comparative non-isotopic in situ hybridization. Wild-type C57BL/6J mice were studied at various stages of embryonic development (from 8.5- to 13.5-day-old embryos - E8.5-13.5). During early odontogenesis, transcripts for Bmp-4, Shh and Wnt-5a were co-localised at the tooth initiation stage. At E8.5, Shh mRNA expression was restricted to diencephalon and pharyngeal endoderm. Before maxillae and mandible ossification, Bmp-4 and Wnt-5a signals were detected in the mesenchymal cells and around Meckel's cartilage. During palatogenesis, Shh was expressed only in the epithelium and Wnt-5a only in the mesenchyme of the elevating palatal shelves. During tongue development, Shh expression was found in mesenchyme, probably contributing to tongue miogenesis, while Wnt-5a signal was in the epithelium, possibly during placode development and papillae formation. Taken together, these findings suggest that Bmp-4, Shh and Wnt-5a gene expressions may act together on the epithelial mesenchymal interactions occurring in several aspects of the early mouse craniofacial development, such as odontogenesis, neuronal development, maxillae and mandible ossification, palatogenesis and tongue formation. (C) 2009 Elsevier GmbH. All rights reserved.