Method and apparatus for characterization of electric field-induced aggregation in pre-crystalline protein solutions
Method and apparatus for characterization of electric field-induced aggregation in pre-crystalline protein solutions
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DOI：
10.1063/1.4906328
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发表时间：
2015-01-01
影响因子：
1.6
通讯作者：
Wakamatsu, Takashi
中科院分区：
文献类型：
--
作者：
Wakamatsu, Takashi
本文提出了一种在外加电场下表征蛋白质聚集的方法和装置。该方法基于前向光散射技术，该技术对预结晶蛋白质溶液中的聚集体高度敏感。透明导电膜被用作平面薄样池的电极，这使得能够精确地测量通过电极的小角度的前向光散射。对一个溶菌酶模型蛋白在外加电场作用下的蛋白质聚集进行了评价。在低电压下对结晶溶菌酶溶液的原位测量表明，前向静态光散射分布随时间变化为幂函数分布。这表明在外加电场的作用下，溶菌酶在晶前状态下形成了分形团簇。本文提出的方法和装置可以灵敏地评价外加电场下蛋白质结晶的促进过程。(C)2015 AIP出版有限责任公司。
The article presents a method and an apparatus for the characterization of protein aggregation under an applied internal electric field. The method is based on a forward light scattering technique that is highly sensitive to aggregates in pre-crystalline protein solutions. Transparent conductive films are used as electrodes for a planar thin sample cell, which enables precise measurement of the forward light scattering at small angles through the electrodes. Evaluation of the protein aggregation under applied electric fields was demonstrated for a model lysozyme protein. In situ measurements of crystallizing lysozyme solutions under a low applied voltage revealed that the forward static light scattering profiles changed with time into power law profiles. This indicates the formation of lysozyme fractal clusters under applied electric fields in the pre-crystalline state. The method and the apparatus presented here can sensitively evaluate the promotion process in protein crystallization under an applied electric field. (C) 2015 AIP Publishing LLC.