A disposable paper-based microfluidic immunosensor based on reduced graphene oxide-tetraethylene pentamine/Au nanocomposite decorated carbon screen-printed electrodes
A disposable paper-based microfluidic immunosensor based on reduced graphene oxide-tetraethylene pentamine/Au nanocomposite decorated carbon screen-printed electrodes
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DOI：
10.1016/j.snb.2017.05.148
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发表时间：
2017-11-01
影响因子：
8.4
通讯作者：
Chen, Zhencheng
中科院分区：
文献类型：
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作者：
Cao, Liangli;Fang, Cheng;Chen, Zhencheng
以纳米金修饰氧化石墨烯-四乙烯五胺(rGO-TEPA/Au纳米复合材料)为电极材料，研制了一种新型、一次性、灵敏的微流控纸基免疫传感器(MU-PEI)。由于具有良好的导电性和较大的比表面积，rGO-TEPA极大地放大了电流响应。为了提高rGO-TEPA/Au纳米复合材料的生物相容性并保持良好的稳定性，将Au纳米颗粒修饰在rGO-TEPA表面，作为锚定捕获抗体(Ab)和加速电子传递到丝网印刷电极(SPE)的有效传感器平台。以rGO-TEPA/Au纳米复合修饰SPE(SPES/rGO-TEPA/Au)为模板，设计了一种简单、一次性的免疫层析和免疫过滤相结合的纸基微流控通道。将便携式SPE/rGO-TEPA/Au和简单的纸基微流控器件相结合，探索了辣根过氧化物酶(HRP)和标记信号抗体(Ab2)共固定的金纳米棒(HRP-GNRs-Ab2)作为方波伏安法(SWV)的示踪剂。以甲胎蛋白为模型分析物，MU-PEI具有制备简单、稳定性高、选择性好、线性范围宽(0.01ngmL(-1)~100.0 ngmL(-1))和低检测限(0.005 ngmL(-1))等特点。此外，将人血清应用于所获得的Mu-PEI，并进行了测定，结果令人满意。(C)2017年，由爱思唯尔出版。
A novel, disposable and sensitive microfluidic paper-based electrochemical immunosensor (mu-PEI) was developed by using gold nanoparticles (Au NPs) decorated reduced graphene oxide-tetraethylene pentamine (rGO-TEPA/Au nanocomposite) as electrode materials. The rGO-TEPA greatly amplified the current response due to its excellent conductivity and large surface area. Au NPs was decorated on the surface of rGO-TEPA to increase the biocompatibility and retained good stability for rGO-TEPA/Au nanocomposite, which was used as an effective sensor platform for anchoring the capturing antibodies (Ab]) and accelerating the electron transfer to the screen-printed electrodes (SPEs). The simple and disposable paper-based microfluidic channel patterned on the rGO-TEPA/Au nanocomposite modified SPEs (SPEs/rGO-TEPA/Au) was designed for combination of immunochromatography and immunofiltration simultaneously. With the combination of portable SPEs/rGO-TEPA/Au and simple paper-based microfluidic devices, horseradish peroxidase (HRP) and labeled signal antibodies (Ab(2)) co-immobilized gold nanorods (HRP-GNRs-Ab(2)) was explored as the tracers for square wave voltammetry (SWV) detection. Using Alpha-Fetoprotein (AFP) as a model analyte, the proposed mu-PEI exhibited a satisfactory performance like simple fabrication, high stability, selectivity, wide linear range (0.01 ng mL(-1) -100.0 ng mL(-1)) with a low detection limit (0.005 ng mL(-1)). Furthermore, human serum were applied to the obtained mu-PEI and determined with satisfactory results. (C) 2017 Published by Elsevier B.V.