Genetic diversity of some wild almonds and related Prunus species revealed by SSR and EST-SSR molecular markers
Genetic diversity of some wild almonds and related Prunus species revealed by SSR and EST-SSR molecular markers
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DOI：
10.1007/s00606-011-0536-x
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发表时间：
2012-01-01
影响因子：
1.9
通讯作者：
Chaparro, Jose
中科院分区：
文献类型：
--
作者：
Rahemi, Alireza;Fatahi, Reza;Chaparro, Jose
扁桃及其近缘野生种广泛分布于中亚和西亚，遗传变异程度较高。这是作物改良遗传多样性的重要来源。本研究利用32对SSR和12对EST-SSR引物对89份扁桃及其它李属植物的遗传多样性进行了分析。大部分的加入（68）收集从伊朗的自然栖息地。SSR引物比EST-SSR引物扩增出更多的等位基因，更有效地区分基因型。结果表明，加入之间的多样性高。观察到的杂合性（Ho）为0.581。Nei多样性指数（He）为0.885，平均等位基因数（na）为34。多态信息含量（PIC）平均值为0.874。平均Fst（F统计指数）为0.271，固定指数（Fis）为0.151。估计的群体（AP）和个人（AI）和个人内（WI）的方差分别为5%，35%和60%，这表明，大部分的变异分布在个人之间，而不是组。栽培杏仁与原产于西亚的P. fenzeliana高度相似，支持这些地区在杏仁驯化中的重要性。在聚类分析中，扁桃组和东方组之间的遗传距离最小。Leptopus和Chameamygdalus部分更远离杏仁比李子。结果还表明，十二蕊属（Lycioides）系列在分类上应更接近于真扁桃组。
Almond and its related wild species, which are widely distributed in Central and West Asia, have high genetic variation. This is an important source of genetic diversity for crop improvement. In this study a set of 32 SSR and 12 EST-SSR primer pairs were used to determine genetic diversity in 89 accessions of almond and other Prunus species. Most of the accessions (68) were collected from natural habitats of Iran. SSR primers amplified higher numbers of alleles than EST-SSR markers and discriminated genotypes more effectively. Results indicated high diversity among accessions. Observed heterozygosity (Ho) was 0.581. Nei's index of diversity (He) and average number of alleles per locus (na) were 0.885 and 34, respectively. The mean value of polymorphism information content (PIC) was 0.874. The average Fst (F-statistics index) was 0.271 and the fixation index (Fis) was 0.151. Estimated variance among putative populations (AP) and individuals (AI) and within individuals (WI) were 5, 35, and 60%, respectively, which revealed that most of the variation was distributed among individuals rather than groups. Cultivated almonds were highly similar to P. fenzeliana, which is native to West Asia, supporting the importance of these regions in almond domestication. In the dendrogram of groups, minimum genetic distance was observed between Amygdalus and Orientalis groups from the Euamygdalus section. The Leptopus and Chameamygdalus sections were more distant from almonds than plums. The results also showed Dodecandra (Lycioides) series should be taxonomically classified closer to the section Euamygdalus.