Drilling Combined with Adipose-derived Stem Cells and Bone Morphogenetic Protein-2 to Treat Femoral Head Epiphyseal Necrosis in Juvenile Rabbits
Drilling Combined with Adipose-derived Stem Cells and Bone Morphogenetic Protein-2 to Treat Femoral Head Epiphyseal Necrosis in Juvenile Rabbits
复制标题
钻孔结合脂肪干细胞和骨形态发生蛋白2治疗幼年兔股骨头骨骺坏死。
DOI：
10.1007/s11596-018-1876-3
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发表时间：
2018-04-01
影响因子：
2.4
通讯作者：
Xiong，Kun
中科院分区：
文献类型：
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作者：
Wang，Zi-li;He，Rong-zhen;Xiong，Kun
本研究旨在评估通过生长板钻孔并使用脂肪源性干细胞（ADSC）和骨形态发生蛋白-2（BMP-2）治疗股骨头骨骺缺血性坏死的效果，这可以在幼年兔中进行。培养第四代溴脱氧尿苷 (BrdU) 标记的 ADSC，用 MTT 进行测定以确定其活力，并用茜素红染料染色以确定其成骨能力。 2月龄健康雄性兔（1.2～1.4 kg，n=45）进行缺血诱导，随机分为5组（A组：动物模型对照组；B组：钻孔组；C组：钻孔+ADSC；D组：钻孔+BMP-2；E组：钻孔+ADSC+BMP-2）。治疗后4、6、10周进行磁共振成像（MRI）、X线成像、苏木精和伊红染色以及BrdU免疫荧光检测。大约 90% 的 ADSC 被 BrdU 标记，并表现出良好的活力和成骨能力。在第6周和第10周时，C组和E组的兔子也观察到了类似的结果。与A组和B组相比，C组和E组的动物表现出正常的髋部结构，股骨骨骺商和小梁面积有所改善（P<0.01）。与A、B组相比，D组股骨骨骺商和小梁面积均有所改善（P<0.05）。总之，在幼年兔股骨头骨骺缺血性坏死模型中，通过生长板钻孔结合 ADSC 和 BMP-2 治疗可诱导新骨形成，并保护股骨头骨骺免于塌陷。
This study was designed to evaluate the effects of drilling through the growth plate and using adipose-derived stem cells (ADSCs) and bone morphogenetic protein-2 (BMP-2) to treat femoral head epiphyseal ischemic necrosis, which can be done in juvenile rabbits. Passagefour bromodeoxyuridine (BrdU)-labeled ADSCs were cultured, assayed with MTT to determine their viability and stained with alizarin red dye to determine their osteogenic ability. Two-month-old, healthy male rabbits (1.2 to 1.4 kg,n=45) underwent ischemic induction and were randomly divided into five groups (group A: animal model control; group B: drilling; group C: drilling & ADSCs; group D: drilling & BMP-2; and group E: drilling & ADSCs & BMP-2). Magnetic resonance imaging (MRI), X-ray imaging, hematoxylin and eosin staining and BrdU immunofluorescence detection were applied 4, 6 and 10 weeks after treatment. Approximately 90% of the ADSCs were labeled with BrdU and showed good viability and osteogenic ability. Similar results were observed in the rabbits in groups C and E at weeks 6 and 10. The animals of groups C and E demonstrated normal hip structure and improved femoral epiphyseal quotients and trabecular areas compared with those of the groups A and B (P<0.01). Group D demonstrated improved femoral epiphyseal quotients and trabecular areas compared with those of groups A and B (P<0.05). In summary, drilling through the growth plate combined with ADSC and BMP-2 treatments induced new bone formation and protected the femoral head epiphysis from collapsing in a juvenile rabbit model of femoral head epiphyseal ischemic necrosis.