REMOVAL AND EXCRETION OF IMMUNOREACTIVE RAT GROWTH-HORMONE BY THE ISOLATED KIDNEY
REMOVAL AND EXCRETION OF IMMUNOREACTIVE RAT GROWTH-HORMONE BY THE ISOLATED KIDNEY
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DOI：
10.1152/ajprenal.1981.240.4.f282
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发表时间：
1981-01-01
影响因子：
--
通讯作者：
FANG, VS
中科院分区：
文献类型：
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作者：
RABKIN, R;GOTTHEINER, TI;FANG, VS
在分离的灌流大鼠肾脏中检测免疫反应性大鼠生长激素(RGH)的摄取，以确定肾小管周围是否发生大于12,000道尔顿的蛋白质的去除，并评估肾GH摄取的功能特征。RGH(OCGH)在对照肾脏的器官清除率为1039.+-。99微升/分钟，不受过量胰岛素的影响，但受寒冷(10度)明显抑制。C)和KCN。尽管对照组大鼠的肾小球滤过率(GFR)与OCGH无显著差异，但由于肾小球蛋白的筛选，滤过不能解释RGH全部被去除。冷和KCN治疗组肾小管滤过率明显低于OCGH组，提示肾小管周围摘除的发生。在非滤过性肾脏，RGH清除量超过[14C]菊粉(P<；0.05)，显示肾小管周围RGH清除量。RGH的肾小管吸收不受胰岛素的影响，但受到寒冷和KCN的明显抑制。RGH主要通过肾小球滤过-肾小管吸收途径从肾循环中清除，但另外，与较小的蛋白质一样，肾小管周围的清除也发生。
The renal uptake of immunoreactive rat growth hormone (rGH), MW 21,500 daltons, was examined in the isolated perfused rat kidney to determine whether peritubular removal of a protein greater than 12,000 daltons occurs and to assess the functional characteristics of renal GH uptake. Organ clearance of rGH (OCGH) in control kidneys was 1039 .+-. 99 .mu.l/min and was unaffected by an excess of insulin but markedly depressed by cold (10.degree. C) and KCN. Although glomerular filtration rate (GFR) did not differ significantly from OCGH in the control rats, it was suspected that filtration could not account for all the rGH removed because of glomerular protein sieving. GFR was significantly less than OCGH with cold and KCN treatment, indicating the occurrence of peritubular removal. In nonfiltering kidneys, rGH removal exceeded that of [14C]inulin (P < 0.05), demonstrating peritubular rGH removal. Tubular absorption of rGH was unaffected by insulin but markedly depressed by cold and KCN. rGH is removed from the renal circulation mainly by the glomerular filtration-tubular absorptive pathway, but in addition, as with smaller proteins, peritubular removal occurs.