Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome
Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome
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DOI：
10.1099/vir.0.81930-0
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发表时间：
2006-09-01
影响因子：
3.8
通讯作者：
Kobayashi, Michihiro
中科院分区：
文献类型：
--
作者：
Ikeda, Motoko;Shikata, Masamitsu;Kobayashi, Michihiro
分析了美国白蛾核型多角体病毒（HycuNPV）的全基因组序列。HycuNPV基因组全长132959 bp，G+C含量为45.1mol%。共148个开放阅读框（ORF），由50个以上氨基酸组成。是由基因组编码的。HycuNPV与其它鳞翅目I组核型多角体病毒（Autographa californica MNPV）、桑尺蠖核型多角体病毒（Atrixmori NPV）、烟卷蛾核型多角体病毒（ChoristoneurafumiferanaMNPV）、烟卷蛾缺陷型核型多角体病毒（ChoristoneurafumiferanaDeficient NPV）、稻纵卷蛾核型多角体病毒（EpiphyaspostvittanaMNPV）和拟杉毒蛾核型多角体病毒（OrgyiapseudotsugataMNPV）等核型多角体病毒共有122个开放阅读框。六个ORF被确定为HycuNPV所特有的。HycuNPV与CfMNPV和OpMNPV的同源性高于其它I组核型多角体病毒。HycuNPV编码两个芋螺毒素样同源物（ctl），其仅在I组NPV中的OpMNPV中观察到。HycuNPV编码三种细胞凋亡抑制剂（iaps），hycu-iap-1、hycu-iap-2和hycu-iap-3，这是它仅与CfMNPV共享的特征。此外，在HycuNPV基因组中鉴定了六个同源区域（hrs）。这些hrs位于与OpMNPV冷杉相似的区域，但与大多数CfMNPV hrs不同。根据HycuNPV与CfMNPV和OpMNPV的亲缘关系和I组NPV特异基因gp 64、ie-2和ptp-1的保守性，认为HycuNPV属于I组NPV，与CfMNPV和OpMNPV最相似。
The whole-genome sequence of the Hyphantria cunea nucleopolyhedrovirus (HycuNPV) was analysed. The entire nucleotide sequence of the HycuNPV genome was 132 959 bp long, with a G+C content of 45.1 mol%. A total of 148 open reading frames (ORFs) consisting of more than 50 aa. were encoded by the genome. HycuNPV shares more than 122 ORFs with other lepidopteran group I NPVs, including Autographa californica MNPV, Bombyx mori NPV, Choristoneura fumiferana MNPV(CfMNPV), Choristoneura fumiferana defective NPV, Epiphyas postvittana MNPV and Orgyia pseudotsugata MNPV(OpMNPV). Six ORFs are identified as being unique to HycuNPV. Most of the HycuNPV ORFs showed higher similarity to CfMNPV and OpMNPV ORFs than to those of the other group I NPVs. HycuNPV encodes two conotoxin-like homologues (ctls), which are observed only in OpMNPV in group I NPVs. HycuNPV encodes three inhibitors of apoptosis (iaps), hycu-iap-1, hycu-iap-2 and hycu-iap-3, a feature that it shares only with CfMNPV. In addition, six homologous regions (hrs) are identified in the HycuNPV genome. These hrs are located in regions similar to those of the OpMNPV firs, but different from most of the CfMNPV hrs. Based on the close phylogenetic; relationship and conservation of group I NPV-specific genes, such as gp64, ie-2 and ptp-1, it is concluded that HycuNPV belongs to the group I NPVs and is most similar to CfMNPV or OpMNPV.