Identity of Cypripedium calceolus (Orchidaceae) in Rebun Island: comparative DNA analysis of related species.
Identity of Cypripedium calceolus (Orchidaceae) in Rebun Island: comparative DNA analysis of related species.
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礼文岛Cypripedium calceolus（兰科）的身份：相关物种的比较DNA 分析。
DOI：
10.1266/ggs.76.181
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发表时间：
2001
影响因子：
1.1
通讯作者：
M. Katsumi
中科院分区：
文献类型：
--
作者：
H. Taniguchi;T. Nakamura;H. Mizukami;S. Kawano;H. Sano;M. Katsumi
Cypripedium calceolus 于 1980 年在日本北海道北海岸的礼文岛被发现，但这种植物的起源一直存在争议。本研究通过叶绿体DNA测序分析，对礼文岛的C. calceolus与西欧、中国和俄罗斯远东（纳霍德卡）的C. calceolus种群，以及日本的C. macranthos和北美的其他杓兰属物种进行了比较研究。杓兰 cf.最近在北海道东部发现的“calceolus”也包含在这次分析中。分析的C. calceolus样本分为三组，即来自西欧、来自中国和俄罗斯远东地区以及来自礼文岛的样本。礼文岛的C. calceolus在DNA序列和形态特征上与其他的有明显不同。 C. cf.来自北海道东部的 calceolus 和来自俄罗斯 Nadhodka 的一个样本也与来自礼文岛的样本属于同一类，尽管它们的形态特征存在一些差异。结论是礼文岛发现的C. calceolus与欧洲和中国生长的C. calceolus并不相同。此外，发现可以将C. macranthos分为两个组，即包括或不包括var. var.的组。雷布嫩塞。在礼文岛发现的一种不明杓兰属物种与变种杓兰属同一组。雷布嫩塞。
Cypripedium calceolus was found in 1980 in Rebun Island off the north coast of Hokkaido, Japan, but the origin of this plant has been a controversial issue. In this study, we have made a comparative study by chloroplast DNA sequencing analysis among C. calceolus which occurs in Rebun Island and populations of C. calceolus from western Europe, China and far eastern Russia (Nakhodka), and also as references, C. macranthos in Japan and other Cypripedium species in North America. A Cypripedium cf. "calceolus", found recently in eastern Hokkaido, was also included in this analysis. The C. calceolus samples analyzed were categorized into three groups, i.e., those from Western Europe, from China and far eastern Russia, and from Rebun Island. The C. calceolus in Rebun Island was clearly different from the others in terms of DNA sequence and morphological features. The C. cf. calceolus from eastern Hokkaido and one sample from Nadhodka, Russia, were also classified into the same group as those from Rebun Island, although some differences in their morphological features were observed. It is concluded that the C. calceolus found in Rebun Island is not identical with those growing in Europe and China. In addition, it was found that it may be possible to classify C. macranthos into two groups, namely groups which include or do not include var. rebunense. An unidentified Cypripedium species found in Rebun Island falls into the same group as var. rebunense.