Modification of a rapid method for the identification of gene-specific polymorphisms in Cryptosporidium parvum and its application to clinical and epidemiological investigations
Modification of a rapid method for the identification of gene-specific polymorphisms in Cryptosporidium parvum and its application to clinical and epidemiological investigations
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DOI：
10.1128/aem.67.12.5581-5584.2001
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发表时间：
2001-12-01
影响因子：
4.4
通讯作者：
Casemore, DP
中科院分区：
文献类型：
--
作者：
Elwin, K;Chalmers, RM;Casemore, DP
隐孢子虫临床分离株基因分型的应用显着增加了我们的知识和了解这种寄生虫的分布和流行病学。然而，有些方法可能很费力，需要专业的技术知识。PCR-限制性片段长度多态性（RFLP）技术是一种比传统DNA分析技术更快速、更简单的基因分型方法，可用于支持流行病学和临床研究。我们描述了一种巢式PCR-RFLP技术，确定多态性的C。该方法为微小梭菌血小板反应蛋白相关粘附蛋白基因位点的检测提供了一种灵敏、特异的方法。小的这种增强的方法的潜力证明了其应用程序的确认和流行病学调查隐孢子虫病的爆发与学校访问一个开放的农场。
The application of genotyping to clinical isolates of Cryptosporidium has increased significantly our knowledge and understanding of the distribution and epidemiology of this parasite. However, some methods can be laborious and demand specialist technical expertise. PCR-restriction fragment length polymorphism (RFLP) techniques represent a more rapid and simple method of genotyping to support epidemiological and clinical investigations than conventional DNA analytical techniques. We describe a nested PCR-RFLP technique that identifies polymorphisms in the C. parvum thrombospondin-related adhesive protein gene locus; this method offers a sensitive and specific tool for the confirmation and investigation of disease associated with C. parvum. The potential of this enhanced method is demonstrated by its application to the confirmation and epidemiological investigation of an outbreak of cryptosporidiosis associated with a school visit to an open farm.