Isolation of Rhodospirillum centenum mutants defective in phototactic colony motility by transposon mutagenesis
Isolation of Rhodospirillum centenum mutants defective in phototactic colony motility by transposon mutagenesis
复制标题
DOI：
10.1128/jb.180.5.1248-1255.1998
复制
发表时间：
1998-03-01
影响因子：
3.2
通讯作者：
Bauer, CE
中科院分区：
文献类型：
--
作者：
Jiang, ZY;Rushing, BG;Bauer, CE
紫色光合细菌centenum红螺旋菌（Rhodospirillum centenum）能够形成快速向光或远离光迁移的群体菌落，这取决于激发的波长。为了确定光敏反应的特定成分，我们进行了mini- tn5介导的诱变，并筛选了大约23,000个转位事件，这些突变体对连续照明或光强减弱都没有反应。250个突变体失去了在琼脂表面形成可移动的群体细胞的能力，这些细胞似乎在合成上存在缺陷。表面诱导的外侧鞭毛组装。另一大部分对光无反应的突变体在形成功能性光合装置方面存在缺陷。还获得了一些光感突变体，它们对光信号的感知和传输存在缺陷。在这类突变体中，有12个突变体含有趋化操纵子的破坏，而其他突变体含有光觉特有成分的破坏。这表明，筛选光觉缺陷的百里氏菌群菌落是研究真细菌8感机制的有效方法。
The purple photosynthetic bacterium Rhodospirillum centenum is capable of forming swarm colonies that rapidly migrate toward or away from light, depending on the wavelength of excitation, To identify components specific for photoperception, we conducted mini-Tn5-mediated mutagenesis and screened approximately 23,000 transposition events for mutants that failed to respond to either continuous illumination or to a step down in light intensity, A majority of the ca, 250 mutants identified lost the ability to form motile swarm cells on an agar surface, These cells appeared to contain defects in the synthesis on. assembly of surface-induced lateral flagella. Another large fraction of mutants that were unresponsive to light were shown to be defective in the formation of a functional photosynthetic apparatus. Several photosensory mutants also were obtained with defects in the perception and transmission of light signals. Twelve mutants in this class were shown to contain disruptions in a chemotaxis operon, and dive mutants contained disruptions of components unique to photoperception, It was shown that screening for photosensory defective R. centenum swarm colonies is ant effective method for genetic dissection of the mechanism of Eight sensing in eubacteria.