Fast start-up of partial nitritation as pre-treatment for anammox in membrane bioreactor
Fast start-up of partial nitritation as pre-treatment for anammox in membrane bioreactor
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DOI：
10.1016/j.bej.2015.10.018
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发表时间：
2016-01-15
影响因子：
3.9
通讯作者：
Kawagoshi, Yasunori
中科院分区：
文献类型：
--
作者：
Huang, Xiaowu;Urata, Kohei;Kawagoshi, Yasunori
厌氧氨氧化是一种新型的生物脱氮技术。原含氮废水由于缺乏足够的亚硝酸盐，不适合厌氧氨氧化直接去除。在膜生物反应器（MBR）中运行部分亚硝化（PN）以产生适合厌氧氨氧化的进料，并通过16 S rRNA序列分析来分析功能微生物。以溶解氧为关键选择因子实现部分亚硝化。在19天内在MBR中实现PN。从第26天开始，部分亚硝化是稳定的，并且在流出物NO2--N/NH 4 +-N比为1.15 +/-0.09的情况下操作，这是适合于供给后续厌氧氨氧化的混合物。游离氨对亚硝酸盐氧化剂的活性有显著的抑制作用。在整个实验过程中达到了94.6 +/- 3.1%的高亚硝酸盐积累效率。生物反应器的最佳溶解氧为0.8-0.9 mg L-1。分子克隆和系统发育分析表明，亚硝化螺菌是MBR中实现亚硝化的优势氨氧化菌。序列已在GenBank中登录，命名为亚硝化螺旋菌YKU。膜生物反应器有望实现一段式部分亚硝化和厌氧氨氧化。(C)2015 Elsevier B. V.版权所有。
Anaerobic ammonium oxidation (anammox) is an innovative pathway for biological nitrogen removal. The raw nitrogenous wastewater is ill-suited for direct depletion by anammox due to lack of sufficient nitrite. Partial nitritation (PN) was operated in a membrane bioreactor (MBR) to generate suitable feed for anammox, and functional microbes were analysed by 16S rRNA sequence analysis. The dissolved oxygen was adopted as the key selection factor to realize partial nitritation. The PN was realized in the MBR within 19 days. From day 26 and onwards, partial nitritation was stable and operated with an effluent NO2--N/NH4+-N ratio of 1.15 +/- 0.09, which was a suitable mixture to feed subsequent anammox. The free ammonia overwhelmingly restrained the activity of nitrite oxidizers. High nitrite accumulation efficiency of 94.6 +/- 3.1% was achieved throughout the experiment. The optimal dissolved oxygen of the bioreactor to create suitable media was 0.8-0.9 mg L-1. The molecular cloning and phylogenetic analysis revealed that Nitrosospira sp. was the predominant ammonium-oxidizing bacteria corresponding to implement nitritation in the MBR. Sequences with original name of Nitrosospira sp. YKU were deposited in GenBank. The MBR promised potential merit to implement one-stage partial nitritation and anammox. (C) 2015 Elsevier B.V. All rights reserved.