Rapid development and plasticity of layer 2/3 maps in rat barrel cortex in vivo
Rapid development and plasticity of layer 2/3 maps in rat barrel cortex in vivo
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DOI：
10.1016/s0896-6273(01)00360-9
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发表时间：
2001-08-02
期刊：
影响因子：
16.2
通讯作者：
Svoboda, K
中科院分区：
文献类型：
--
作者：
Stern, EA;Maravall, M;Svoboda, K
皮层突触回路在出生后第二周迅速发育，同时树突棘的经验依赖性周转。为了将感觉图谱的出现与突触发生联系起来，我们记录了出生后12至20天2/3层神经元中须偏转引起的突触电位。在P12，突触反应无法检测到。仅在2天后（P14），接受野就有了成熟的组织。感觉剥夺，如果在P14之前开始，会破坏感受野结构。在第4层，反应和地图在P12时已经成熟，对剥夺不敏感，说明桶状皮层从第4层发育到第2/3层。因此，P12-14是2/3层突触及其棘共享的关键时期，这表明棘的可塑性参与了图谱的完善。
Cortical synaptic circuitry develops rapidly in the second postnatal week, simultaneous with experience-dependent turnover of dendritic spines. To relate the emergence of sensory maps to synaptogenesis, we recorded synaptic potentials evoked by whisker deflection in layer 2/3 neurons from postnatal day (P) 12 to 20. At P12, synaptic responses were undetectable. Only 2 days later in life (P14), receptive fields had mature organization. Sensory deprivation, if initiated before P14, disrupted receptive field structure. In layer 4, responses and maps were already mature by P12 and insensitive to deprivation, implying that barrel cortex develops from layer 4 to layer 2/3. Thus, P12-14 is a critical period shared by layer 2/3 synapses and their spines, suggesting that spine plasticity is involved in the refinement of maps.