VOLTAGE-DEPENDENT CALCIUM CHANNELS IN SMOOTH-MUSCLE CELLS
VOLTAGE-DEPENDENT CALCIUM CHANNELS IN SMOOTH-MUSCLE CELLS
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DOI：
10.1097/00005344-198812006-00003
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发表时间：
1988-01-01
影响因子：
3
通讯作者：
AARONSON, PI
中科院分区：
文献类型：
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作者：
BOLTON, TB;MACKENZIE, I;AARONSON, PI
大约20年前提出平滑肌细胞中的动作电位是由于Ca 2+而不是Na+离子的流入（Nonomura等人，1966年; Brading等人，1969年）。在20世纪70年代，使用应用于小条平滑肌的电压钳技术研究了负责动作电位上行的Ca 2+电流。这些由许多电池组成，电压控制是可疑的（博尔顿等人，1981）直到使用单细胞（沃尔什和辛格，1981;博尔顿等人，1985年）。将膜片钳应用于单个平滑肌细胞（博尔顿等人，1985）已经变得普遍，并且已经在通过平滑肌组织的急性酶消化获得的单细胞中获得了关于内向电流的大量信息。许多研究者也将细胞培养不同的时间，或者使用平滑肌细胞系。如果细胞被培养，它们的特征可能不是母体组织的特征。很明显，与整个组织相比，即使是急性分散的细胞也会改变它们的反应，因此将结果外推到整个组织需要一些注意;在分离的细胞中，一些机制可能会丢失，而其他机制可能会被掩盖或夸大。
It was suggested about 20 years ago that the action potential in smooth muscle cells was due to an influx of Ca²+ rather than Na+ ions (Nonomura et al., 1966; Brading et al., 1969). In the 1970s, the Ca2+ current responsible for the upstroke of the action potential was studied using the voltage-clamp technique applied to small strips of smooth muscle. These consisted of many cells and voltage control was suspect (Bolton et al., 1981) until the use of single cells (Walsh and Singer, 1981; Bolton et al., 1985). Applying a patch clamp to single smooth muscle cells (Bolton et al., 1985) has now become commonplace and considerable information concerning the inward current has been obtained in single cells which have been obtained by acute enzymic digestion of smooth muscle tissues. Cells have also been maintained in culture for varying periods by many investigators, or smooth muscle cell lines have been used. If cells are cultured, their characteristics may not be those of the parent tissue. It is clear that even acutely dispersed cells can be altered in their responses compared to the whole tissue, so that extrapolation of the results to the whole tissue needs some care; some mechanisms may be lost in isolated cells and others may be unmasked or exaggerated.