Changes in ABCB1 mRNA Expression in Peripheral Blood Cells before and after Renal Transplantation
Changes in ABCB1 mRNA Expression in Peripheral Blood Cells before and after Renal Transplantation
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DOI：
10.1248/bpb.b15-00690
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发表时间：
2016-07-01
影响因子：
2
通讯作者：
Kobayashi, Takaaki
中科院分区：
文献类型：
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作者：
Kushihara, Hideyuki;Kuzuya, Takafumi;Kobayashi, Takaaki
环孢素 (CSA) 是 ATP 结合盒亚家族 B 成员 1 (ABCB1) 的底物之一，广泛用作移植患者的免疫抑制剂。 ABCB1基因编码的淋巴细胞上P-糖蛋白的表达水平被认为是细胞内CSA浓度差异的主要原因之一。分析外周血ABCB1 mRNA表达的临床相关性。我们检查了（i）体外ABCB1 mRNA与CSA细胞内浓度之间的关系，（ii）长期ABCB1 mRNA表达水平的变化，以及（iii）其与活体肾移植中急性排斥（AR）或巨细胞病毒（CMV）再激活的关系。 ABCB1 mRNA 表达与体外细胞内 CSA 浓度呈显着负相关（p
Cyclosporine (CSA), which is one of the substrates of ATP binding cassette subfamily B member 1 (ABCB1), is widely used as an immunosuppressant in patients undergoing transplantation. The expression level of P-glycoprotein on lymphocytes that is encoded by ABCB1 gene is considered to be one of the major causes of differences in intracellular CSA concentration. The clinical relevance of ABCB1 mRNA expression in peripheral blood was analyzed. We examined (i) the relationship between ABCB1 mRNA and the intracellular concentration of CSA in vitro, (ii) the change in long-term ABCB1 mRNA expression levels, and (iii) its association with acute rejection (AR) or cytomegalovirus (CMV) reactivation in living-donor renal transplantation. A significantly negative correlation between ABCB1 mRNA expression and intracellular CSA concentration in vitro was obtained (p