Role of outer surface protein D in the Borrelia burgdorferi life cycle
Role of outer surface protein D in the Borrelia burgdorferi life cycle
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DOI：
10.1128/iai.00632-07
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发表时间：
2007-09-01
影响因子：
3.1
通讯作者：
Fikrig, Erol
中科院分区：
文献类型：
--
作者：
Li, Xin;Neelakanta, Girish;Fikrig, Erol
伯氏疏螺旋体优先诱导小鼠或蜱中的选定基因，研究表明 ospD 因宿主特异性信号而下调。我们现在直接表明，与小鼠相比，肩胛硬蜱中的 ospD 表达通常升高 14。然后，我们通过生成 OspD 缺陷的伯氏疏螺旋体并检查蜱传播的莱姆疏螺旋体病小鼠模型中的突变体，评估了 OspD 在整个螺旋体生命周期中的重要性。 OspD 的缺乏并不影响小鼠中伯氏疏螺旋体的感染性或肩胛伊氏菌获得螺旋体。在体外，OspD 粘附在蜱肠道提取物上，而 OspD 缺陷的伯氏疏螺旋体菌株在体内蜱肠道的定植减少了三倍。然而，这种减少并没有改变第二次吸血期间随后的螺旋体传播。这些数据表明，伯氏疏螺旋体可以补偿蜱和小鼠中OspD的缺乏，并且OspD可能在肩胛疏螺旋体中的伯氏疏螺旋体持久性中具有非必要的、次要的作用。
Borrelia burgdorferi preferentially induces selected genes in mice or ticks, and studies suggest that ospD is down-regulated in response to host-specific signals. We now directly show that ospD expression is generally 14 elevated within Ixodes scapularis compared with mice. We then assessed the importance of OspD throughout the spirochete life cycle by generating OspD-deficient B. burgdorferi and examining the mutant in the murine model of tick-transmitted Lyme borreliosis. The lack of OspD did not influence B. burgdorferi infectivity in mice or the acquisition of spirochetes by I. scapularis. OspD adhered to tick gut extracts in vitro, and the OspD-deficient B. burgdorferi strain had a threefold decrease in colonization of the tick gut in vivo. This decrease, however, did not alter subsequent spirochete transmission during a second blood meal. These data suggest that B. burgdorferi can compensate for the lack of OspD in both ticks and mice and that OspD may have a nonessential, secondary, role in B. burgdorferi persistence within I. scapularis.