PEPTIDES FROM MYELIN BASIC-PROTEIN AS SUBSTRATES FOR ADENOSINE 3', 5'-CYCLIC MONOPHOSPHATE-DEPENDENT PROTEIN KINASES
PEPTIDES FROM MYELIN BASIC-PROTEIN AS SUBSTRATES FOR ADENOSINE 3', 5'-CYCLIC MONOPHOSPHATE-DEPENDENT PROTEIN KINASES
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DOI：
10.1016/0006-291x(74)90234-4
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发表时间：
1974-01-01
影响因子：
3.1
通讯作者：
CARNEGIE, PR
中科院分区：
文献类型：
--
作者：
DAILE, P;CARNEGIE, PR
简单的电泳测定表明，人髓磷脂碱性蛋白酶消化得到的肽是牛心肌和脑中腺苷 3', 5'-环单磷酸依赖性蛋白激酶的有效底物。从胃消化物中分离出 17 个氨基酸残基的肽，当用作底物时，发现了心肌激酶的 Kmof 1.9 × 10−4M。
A simple electrophoretic assay demonstrated that peptides from enzymic digests of the basic protein of human myelin were effective substrates for adenosine 3′, 5′-cyclic monophosphate-dependent protein kinases from bovine cardiac muscle and brain. From a peptic digest a peptide of 17 amino acid residues was isolated and when used as a substrate a Kmof 1.9 × 10−4M was found for the cardiac kinase.