Electrospun Silk Fibroin-Hydroxybutyl Chitosan Nanofibrous Scaffolds to Biomimic Extracellular Matrix
Electrospun Silk Fibroin-Hydroxybutyl Chitosan Nanofibrous Scaffolds to Biomimic Extracellular Matrix
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静电纺丝素-羟丁基壳聚糖纳米纤维支架仿生细胞外基质
DOI：
10.1163/092050610x498204
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发表时间：
2011-01-01
影响因子：
3.6
通讯作者：
Mo, Xiumei
中科院分区：
文献类型：
--
作者：
Zhang, Kuihua;Qian, Yongfang;Mo, Xiumei
以1，1，1，3，3，3-六氟-2-丙醇(HFIP)和三氟乙酸(TFA)为溶剂，通过静电纺丝仿生天然ECM，制备了丝素蛋白(SF)-羟丁基壳聚糖(HBC)共混纳米纤维支架。扫描电子显微镜结果表明，当HBC的含量从20%增加到100%时，BE的平均纳米纤维直径增大。而水接触角的测量证实了不同重量比的SF/HBC纳米纤维支架具有良好的亲水性。当SF与HBC的质量比为20：80时，拉伸强度和断裂伸长率均有明显提高。~(13)C-核磁共振证实SF和HBC分子以氢键相互作用的形式存在，但HBC不能诱导SF构象从无规卷曲结构向β-折叠结构转变。此外，京尼平蒸气的使用不仅诱导SF的构象从无规卷曲转变为β-折叠结构，而且还作为SF和HBC的交联剂。细胞活性研究表明，SF/HBC纳米纤维支架具有良好的细胞相容性。因此，电纺SF/HBC共混纳米纤维可能提供一种理想的仿生组织工程支架。(C)Koninklijke Brill NV，莱顿，2011年
Silk fibroin (SF) hydroxybutyl chitosan (HBC) blend nanofibrous scaffolds were fabricated using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and trifluoroacetic acid (TFA) as solvents to biomimic the native ECM by electrospinning. SEM results showed that be average nanofibrous diameter increased when the content of HBC was raised from 20% to 100%. Whereas water contact angle measurements confirmed that SF/HBC nanofibrous scaffolds with different weight ratios were of good hydrophilicity. Both the tensile strength and the elongation at break were improved obviously when the weight ratio of SF to HBC was 20:80. C-13-NMR clarified that SF and HBC molecules existed in H-bond interactions, but HBC did not induce SF conformation to transform from random coil form to beta-sheet structure. Moreover, the use of genipin vapour not only induced conformation of SF to convert from random coil to beta-sheet structure but also acted as a cross-linking agent for SF and HBC. Cell viability studies demonstrated that SF/HBC nanofibrous scaffolds presented good cellular compatibility. Thus, electrospun SF/HBC blended nanofibres may provide an ideal biomimic tissue-engineering scaffold. (c) Koninklijke Brill NV, Leiden, 2011