Blood testing at the single cell level using quantitative phase and amplitude microscopy.
Blood testing at the single cell level using quantitative phase and amplitude microscopy.
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DOI：
10.1364/boe.2.003259
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发表时间：
2011-12-01
影响因子：
3.4
通讯作者：
Popescu G
中科院分区：
文献类型：
--
作者：
Mir M;Tangella K;Popescu G
最近已经表明，定量相位成像方法可以提供临床相关的参数，红细胞分析前所未有的细节和灵敏度。由于定量相位信息取决于厚度和折射率，因此临床翻译的主要限制是同时测量两者的简单实用方法。在这里，我们从理论上和实验上证明，通过将定量相位与单个吸收测量相结合，可以在单细胞水平上测量这两个量。我们通过将我们的结果与使用临床血液分析仪获得的结果进行比较来验证这种方法。这种将红细胞的厚度和折射率解耦的方法可以与任何定量相位成像方法一起使用，所述定量相位成像方法可以在Soret波段波长下与明场显微镜串联操作。
It has recently been shown that quantitative phase imaging methods can provide clinically relevant parameters for red blood cell analysis with unprecedented detail and sensitivity. Since the quantitative phase information is dependent on both the thickness and refractive index, a major limitation to clinical translation has been a simple and practical approach to measure both simultaneously. Here we demonstrate both theoretically and experimentally that, by combining quantitative phase with a single absorption measurement, it is possible to measure both quantities at the single cell level. We validate this approach by comparing our results to those acquired using a clinical blood analyzer. This approach to decouple the thickness and refractive index for red blood cells may be used with any quantitative phase imaging method that can operate in tandem with bright field microscopy at the Soret-band wavelength.