Molecular characterization of Capripox viruses obtained from field outbreaks in Nigeria between 2000 and 2016
Molecular characterization of Capripox viruses obtained from field outbreaks in Nigeria between 2000 and 2016
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DOI：
10.1111/tbed.13197
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发表时间：
2019-07-01
影响因子：
4.3
通讯作者：
Hoffmann, Bernd
中科院分区：
文献类型：
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作者：
Adedeji, Adeyinka Jeremy;Moeller, Janika;Hoffmann, Bernd
羊痘病毒感染是尼日利亚牲畜的地方性疾病，但关于流行病毒的分子特征的数据有限。在这项研究中，我们调查了现场暴发的羊痘病毒感染在尼日利亚通过部分测序的病毒从现场样品。使用我们新开发的部分测序方案，对2000-2016年从尼日利亚三个州的牛（9）、绵羊（1）和山羊（1）中收集的11份经PCR和实时qPCR检测为羊痘病毒基因组阳性的选定样本进行了表征。这种用于羊痘病毒株遗传特征鉴定的方法允许对该国调查的现场暴发的病毒株进行第一次简短的分子分类。从系统发生学上讲，2010年至2016年获得的8个LSDV样本与2015年和2016年分别在希腊和塞尔维亚发生的已发表菌株密切相关，而2000年的分离株与南非NI-2490菌株高度相似。这些数据表明，在2000年至2010年期间，在尼日利亚传播的LSDV毒株发生了变化。不同年份从山羊和绵羊分离的样品似乎与已知的GTPV毒株有关，但与目前所有已发表的GTPV毒株明显不同。有趣的是，两种新检测到的GTPV毒株彼此之间显示出高达100%的相似性，并导致绵羊和山羊的临床疾病。众所周知，GTPV和SPPV的一些毒株能够感染绵羊和山羊，但在大多数情况下，仅在这些物种中的一种中导致更严重的疾病。这些分离物的进一步遗传特征可以提供更多的洞察，尤其是GTPV和SPPV的Capripox病毒的致病性和毒力因子。
Capripox virus infections are endemic diseases of livestock in Nigeria, but there are limited data on molecular characterization of circulating viruses. In this study, we investigated field outbreaks of Capripox virus infections in Nigeria via partial sequencing of viruses obtained from field samples. Eleven selected samples, collected from 2000-2016 from cattle (9), sheep (1) and goat (1) in three states in Nigeria and Capripox virus genome positive by PCR and real-time qPCR, were characterized using our newly developed partial sequencing protocol. This method for genetic characterization of Capripox virus strains allows a first, short molecular classification of strains responsible for the investigated field outbreaks in the country. Phylogenetically, the eight LSDV samples obtained from 2010 to 2016 are closely related to already published strains occurring in Greece and Serbia in the years 2015 and 2016, respectively, whereas the isolate from 2000 shows high similarity to the South African NI-2490 strain. These data indicate that there was a change of LSDV strains circulating in Nigeria between the years 2000 and 2010. The samples isolated from a goat and a sheep in different years seem to be related to already known GTPV strains, but clearly differ from all current published GTPV strains. Interestingly, both newly detected GTPV strains show up to 100% similarity compared to each other and led to clinical disease in sheep and goats. It is long known that some strains of GTPV and SPPV are able to infect both sheep and goats, but in most cases lead to more severe disease in only one of these species. Further genetic characterization of these isolates could provide more insight into pathogenesis and virulence factors of Capripox viruses, especially GTPV and SPPV.