KNOX1 is expressed and epigenetically regulated during in vitro conditions in Agave spp
KNOX1 is expressed and epigenetically regulated during in vitro conditions in Agave spp
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DOI：
10.1186/1471-2229-12-203
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发表时间：
2012-11-05
影响因子：
5.3
通讯作者：
Robert-Diaz, Manuel L.
中科院分区：
文献类型：
--
作者：
De-la-Pena, Clelia;Nic-Can, Geovanny;Robert-Diaz, Manuel L.
背景：微繁殖是扩大具有经济和农学重要性的植物，提高作物产量的有力工具。然而，一个小的，但越来越多的证据表明，表观遗传机制，如DNA甲基化和组蛋白修饰，可以影响下，在体外条件下的微繁殖的特点。在这里，我们测试了对不同体外系统（洋红盒和生物反应器）的适应是否改变了龙舌兰和龙舌兰的表观遗传学上不同的克隆。狭叶此外，我们评估这些表观遗传变化是否影响调控表达的KNOTTED1样HOMEOBOX（KNOX）transcription factors.Results：为了更好地了解表观遗传变化在体外和离体条件下，龙舌兰fourcroydes和A。为了研究沙枣中的DNA甲基化，我们在两种不同的系统中分析了全局DNA甲基化以及不同的组蛋白修饰标记：半固体在洋红盒中（M）和暂时浸没在模块化生物反应器中（B）。在A.在M或B中生长的四种植物。然而，当A. fourcroydes与A. angustifolia，有一个两倍的物种之间的DNA甲基化的差异，在体外系统中使用的独立。此外，我们在较早在M或B中培养的植物中在离体条件下检测到抑制性标记H3 K9me2的缺失或少量。此外，AtqKNOX 1和AtqKNOX 2在A. fourcroydes和A. angustifolia克隆，在体外条件下受到影响。因此，我们使用染色质免疫沉淀（ChIP）来了解这些基因是否受到表观遗传学调控。与AtqKNOX 2相比，AtqKNOX 1对H3K4me3和H3K9me2的影响更大。结论：在离体条件下，甲基化程度较高的龙舌兰克隆植株更能适应离体条件。此外，A. fourcroydes和A. angustifolia克隆在体外条件下显示出KNOX 1基因的差异表达，这是由H3K4me3和H3K9me2标记的表观遗传调控。在关键发育基因中发现表观遗传调控将使其在未来的研究中变得重要，以确定有助于找到耐气候微繁殖植物的因素。
Background: The micropropagation is a powerful tool to scale up plants of economical and agronomical importance, enhancing crop productivity. However, a small but growing body of evidence suggests that epigenetic mechanisms, such as DNA methylation and histone modifications, can be affected under the in vitro conditions characteristic of micropropagation. Here, we tested whether the adaptation to different in vitro systems (Magenta boxes and Bioreactors) modified epigenetically different clones of Agave fourcroydes and A. angustifolia. Furthermore, we assessed whether these epigenetic changes affect the regulatory expression of KNOTTED1-like HOMEOBOX (KNOX) transcription factors.Results: To gain a better understanding of epigenetic changes during in vitro and ex vitro conditions in Agave fourcroydes and A. angustifolia, we analyzed global DNA methylation, as well as different histone modification marks, in two different systems: semisolid in Magenta boxes (M) and temporary immersion in modular Bioreactors (B). No significant difference was found in DNA methylation in A. fourcroydes grown in either M or B. However, when A. fourcroydes was compared with A. angustifolia, there was a two-fold difference in DNA methylation between the species, independent of the in vitro system used. Furthermore, we detected an absence or a low amount of the repressive mark H3K9me2 in ex vitro conditions in plants that were cultured earlier either in M or B. Moreover, the expression of AtqKNOX1 and AtqKNOX2, on A. fourcroydes and A. angustifolia clones, is affected during in vitro conditions. Therefore, we used Chromatin ImmunoPrecipitation (ChIP) to know whether these genes were epigenetically regulated. In the case of AtqKNOX1, the H3K4me3 and H3K9me2 were affected during in vitro conditions in comparison with AtqKNOX2.Conclusions: Agave clones plants with higher DNA methylation during in vitro conditions were better adapted to ex vitro conditions. In addition, A. fourcroydes and A. angustifolia clones displayed differential expression of the KNOX1 gene during in vitro conditions, which is epigenetically regulated by the H3K4me3 and H3K9me2 marks. The finding of an epigenetic regulation in key developmental genes will make it important in future studies to identify factors that help to find climate-resistant micropropagated plants.