cis-Dimer formation of E-cadherin is independent of cell-cell adhesion assembly in vivo.
cis-Dimer formation of E-cadherin is independent of cell-cell adhesion assembly in vivo.
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E-钙粘蛋白的顺式二聚体形成不依赖于体内细胞-细胞粘附组装。
DOI：
10.1016/j.bbrc.2004.02.123
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发表时间：
2004
影响因子：
3.1
通讯作者：
H. Takeda
中科院分区：
文献类型：
--
作者：
H. Takeda
基于钙粘蛋白的细胞间粘附在胚胎发育和正常组织结构的维持中发挥着重要作用。然而，人们对细胞表面钙粘蛋白动力学的控制机制知之甚少。我们之前证明 E-钙粘蛋白通过化学交联在细胞表面作为顺式（侧向）二聚体发挥作用。在这项研究中，我们通过化学交联研究了细胞-细胞粘附组装过程中 E-钙粘蛋白顺式二聚体形成的动态。尽管用细胞松弛素 D（肌动蛋白聚合的有效抑制剂）治疗可抑制细胞间接触的形成，但 E-钙粘蛋白顺式二聚体形成的动态并未受到影响。该结果表明顺式二聚体的形成过程与体内细胞-细胞粘附组装无关。然而，细胞聚集和解离测定表明，细胞松弛素 D 处理将基于钙粘蛋白的细胞粘附从强状态转变为弱状态。综上所述，这些结果有力地支持了 E-钙粘蛋白顺式二聚体是钙粘蛋白介导的体内细胞间粘附的最小功能单位的可能性。
Cadherin-based cell–cell adhesions play important roles in embryonic development and in the maintenance of normal tissue architecture. Little is known, however, about the mechanisms of controlling cadherin dynamics at the cell surface. We previously demonstrated that E-cadherin functions as a cis (lateral)-dimer on the cell surface by chemical cross-linking. In this study, we examined the dynamics of E-cadherin cis-dimer formation during cell–cell adhesion assembly by using chemical cross-linking. Although treatment with cytochalasin D, a potent inhibitor of actin polymerization, was shown to inhibit the formation of cell–cell contacts, the dynamics of E-cadherin cis-dimer formation was not affected. This result indicated that the cis-dimer formation procedure is independent of cell–cell adhesion assembly in vivo. However, the cell aggregation and dissociation assays showed that the cytochalasin D treatment shifted the cadherin-based cell adhesion from a strong to a weak state. Taken together, these results strongly support the possibility that the E-cadherin cis-dimer is a minimal functional unit in cadherin-mediated cell–cell adhesion in vivo.
DOI：
10.1073/pnas.86.18.7043
发表时间：
1989-09-01
影响因子：
11.1
作者：
FRIEDLANDER, DR;MEGE, RM;EDELMAN, GM
通讯作者：
EDELMAN, GM