Bufei Jianpi granules improve skeletal muscle and mitochondrial dysfunction in rats with chronic obstructive pulmonary disease.
Bufei Jianpi granules improve skeletal muscle and mitochondrial dysfunction in rats with chronic obstructive pulmonary disease.
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补肺健脾颗粒改善慢性阻塞性肺疾病大鼠骨骼肌和线粒体功能障碍
DOI：
10.1186/s12906-015-0559-x
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发表时间：
2015-03-10
影响因子：
--
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Dong Y;Li Y;Sun Y;Mao J;Yao F;Tian Y;Wang L;Li L;Li S;Li J
背景补肺健脾颗粒对肺脾气虚型慢性阻塞性肺疾病（COPD）患者有明显改善肺功能、缓解急性加重期、提高6分钟步行距离和生活质量的作用，并在12个月随访中获益。骨骼肌功能障碍（SMD）是COPD的重要肺外并发症，发生于COPD的初始阶段，与发病率和死亡率密切相关。方法将72只SD大鼠随机分为对照组、补肺健脾组、氨茶碱组、COPD组、COPD +生理盐水组、COPD +补肺健脾组和COPD +氨茶碱组。第9 ~ 20周分别灌胃生理盐水、补肺健脾颗粒和氨茶碱。采用电生理技术检测大鼠肋间肌、股四头肌、肱二头肌和比目鱼肌的肌张力和疲劳指数。采用光镜、电镜和免疫印迹法观察各组大鼠骨骼肌组织病理学和超微结构变化，以及线粒体Bcl-2 19千道尔顿相互作用蛋白3（Bnip 3）和细胞质细胞色素C（Cyto C）的表达。COPD +生理盐水组肌张力、线粒体体密度（Vv）和比较膜表面积（δm）均低于对照组，而疲劳指数、线粒体表面积（δ）、Bnip 3和Cyto C均高于对照组（P < 0.05）。COPD大鼠肌肉组织出现萎缩、变性、坏死及基质增生等形态学改变。超微结构上，四种肌肉中线粒体数量明显减少，萎缩，甚至出现空泡化。结论补肺健脾颗粒通过改善骨骼肌线粒体数量和功能，减少凋亡因子Bnip 3和Cyto C的表达，改善骨骼肌功能，其作用优于氨茶碱。
BackgroundBufei Jianpi granules has been confirmed effective in improving pulmonary function, alleviating acute exacerbations, improving six-minute walk distance and quality of life, and benefited in 12-month follow-up in chronic obstructive pulmonary disease (COPD) patients with syndrome of lung-spleen qi deficiency. Skeletal muscle dysfunction (SMD), an important extrapulmonary complication, occurs in the very initiation of COPD and is closely related to morbidity and mortality. To evaluate the efficacy of Bufei Jianpi granules on SMD, we observed skeletal muscular function and histomorphology, mitochondrial morphormetry and proteins in COPD rats induced by cigarette-smoke andKlebsiellapneumoniae.MethodsSeventy-two Sprague–Dawley rats were randomized into Control + Saline, Control + Bufei Jianpi, Control + Aminophylline, COPD + Saline, COPD + Bufei Jianpi and COPD + Aminophylline groups. From week 9 to 20, rats were administrated intragastricly by normal saline, Bufei Jianpi granules and aminophylline, respectively. Muscular tension and fatigue index of intercostal muscle, quadriceps, biceps and soleus were detected by using electrophysiological technology. Pathological and ultrastructural changes and expressions of mitochondrial Bcl-2 nineteen-kilodalton interacting protein 3 (Bnip3) and cytoplasm cytochrome C (Cyto C) in the four skeletal muscles were observed by using optical and electron microscope and western blotting.ResultsThere was no statistical difference among the control rats treated with saline, Bufei Jianpi granules or aminophylline in above-mentioned parameters. Muscular tension, mitochondria volume density (Vv) and compared membrane surface (δm) of the four muscles were significantly lower in COPD + Saline group compared to Control + Saline group, while fatigue index, mitochondria surface area (δ), Bnip3 and Cyto C were higher (P < 0.05). COPD rats showed more morphological changes in muscle tissues than controls, such as atrophy, degeneration, necrosis and matrix hyperplasia. Utrastructurally, mitochondria populations decreased significantly in the four muscles, and were shrunken and even cavitation changed. The up-mentioned parameters were improved in Bufei Jianpi group (P < 0.05) in the four muscles.ConclusionsBufei Jianpi granules can improve skeletal muscle function via improving mitochondria population and function, reducing apoptotic factors such as Bnip3 and Cyto C, and is more effective than aminophylline.
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影响因子：
--
作者：
Li Y;Li JS;Li WW;Li SY;Tian YG;Lu XF;Jiang SL;Wang Y
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影响因子：
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