Assessment of the Therapeutic Potential of Persimmon Leaf Extract on Prediabetic Subjects
Assessment of the Therapeutic Potential of Persimmon Leaf Extract on Prediabetic Subjects
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DOI：
10.14348/molcells.2017.2298
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发表时间：
2017-07-01
影响因子：
3.8
通讯作者：
Goo, Young Ah
中科院分区：
文献类型：
--
作者：
Khan, Mohd M.;Bao Quoc Tran;Goo, Young Ah
膳食补充剂对人类健康状况表现出无数的积极健康作用，并且随着新技术进步的出现，包括在蛋白质组学、基因组学和代谢组学领域，正在评估膳食补充剂的生物和药理学活性对人类疾病的改善作用。最近的兴趣在理解和发现的分子目标的植物化学基因蛋白质代谢动力学导致发现一些蛋白质签名候选人，可能被用来评估膳食补充剂对人类健康的影响。柿树（Diospyros kaki）是一种民间药物，由于其不同的有益健康作用，包括抗糖尿病作用，在中国，日本和韩国通常用作膳食补充剂。然而，尚未发现可以验证或用于评估柿子对人类健康影响的作用机制或分子生物标志物。在本研究中，基于质谱（MS）的蛋白质组学研究完成，以发现蛋白质组学分子特征，可用于了解柿叶提取物（PLE）在改善糖尿病的治疗潜力。唾液，血清和尿液样本进行了分析，我们建议，唾液蛋白可用于评估治疗效果和提高患者的依从性。本发现蛋白质组学研究表明，在糖尿病前期受试者中发现了作为PLE治疗的结果的唾液蛋白质组学谱变化，其可以特异性地用作潜在的蛋白质特征候选物。
Dietary supplements have exhibited myriads of positive health effects on human health conditions and with the advent of new technological advances, including in the fields of proteomics, genomics, and metabolomics, biological and pharmacological activities of dietary supplements are being evaluated for their ameliorative effects in human ailments. Recent interests in understanding and discovering the molecular targets of phytochemical-gene-protein-metabolite dynamics resulted in discovery of a few protein signature candidates that could potentially be used to assess the effects of dietary supplements on human health. Persimmon (Diospyros kaki) is a folk medicine, commonly used as dietary supplement in China, Japan, and South Korea, owing to its different beneficial health effects including anti-diabetic implications. However, neither mechanism of action nor molecular biomarkers have been discovered that could either validate or be used to evaluate effects of persimmon on human health. In present study, Mass Spectrometry (MS)-based proteomic studies were accomplished to discover proteomic molecular signatures that could be used to understand therapeutic potentials of persimmon leaf extract (PLE) in diabetes amelioration. Saliva, serum, and urine samples were analyzed and we propose that salivary proteins can be used for evaluating treatment effectiveness and in improving patient compliance. The present discovery proteomics study demonstrates that salivary proteomic profile changes were found as a result of PLE treatment in prediabetic subjects that could specifically be used as potential protein signature candidates.