The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster
The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster
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DOI：
10.3791/55024
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发表时间：
2017-03-01
影响因子：
1.2
通讯作者：
Scholz, Henrike
中科院分区：
文献类型：
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作者：
Diegelmann, Soeren;Jansen, Annika;Scholz, Henrike
对于大多数动物来说，进食是确保生存的重要行为，它影响发育、运动、健康和繁殖。因此，摄入正确的食物种类和数量对生活质量有着重大影响。摄食行为的研究集中在确保实际摄食的基本过程，并揭示了调节内部能量稳态的因素和决策的神经基础的作用。模式生物黑腹果蝇（Drosophila melanogaster）具有多种遗传可追溯的标记和操纵单个神经元的工具，可以绘制神经元网络并识别参与食物摄入调节的分子信号级联。本报告演示了CApillary FEeder检测（CAFE），并展示了如何测量一组苍蝇的食物摄入量，时间跨度从数小时到数天不等。这款易于使用的检测试剂盒由玻璃毛细管组成，毛细管中充满了苍蝇可以自由进入和进食的液体食物。检测试剂盒中的食物消耗量可通过简单的测量工具准确测定。在这里，我们描述了一步一步的方法，从设置到成功执行的CAFE测定，并提供了实际的例子来分析一组苍蝇在受控条件下的食物摄入量。读者通过该测定的可能限制以及该方法与D.评价黑腹动物。
For most animals, feeding is an essential behavior for securing survival, and it influences development, locomotion, health and reproduction. Ingestion of the right type and quantity of food therefore has a major influence on quality of life. Research on feeding behavior focuses on the underlying processes that ensure actual feeding and unravels the role of factors regulating internal energy homeostasis and the neuronal bases of decision-making. The model organism Drosophila melanogaster, with its great variety of genetically traceable tools for labeling and manipulating single neurons, allows mapping of neuronal networks and identification of molecular signaling cascades involved in the regulation of food intake. This report demonstrates the CApillary FEeder assay (CAFE) and shows how to measure food intake in a group of flies for time spans ranging from hours to days. This easy-to-use assay consists of glass capillaries filled with liquid food that flies can freely access and feed on. Food consumption in the assay is accurately determined using simple measurement tools. Herein we describe step-by-step the method from setup to successful execution of the CAFE assay, and provide practical examples to analyze the food intake of a group of flies under controlled conditions. The reader is guided through possible limitations of the assay, and advantages and disadvantages of the method compared to other feeding assays in D. melanogaster are evaluated.