Association between equine temperament and polymorphisms in dopamine D4 receptor gene
Association between equine temperament and polymorphisms in dopamine D4 receptor gene
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DOI：
10.1007/s00335-005-0021-3
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发表时间：
2005-07-01
期刊：
影响因子：
2.5
通讯作者：
Mori, Y
中科院分区：
文献类型：
--
作者：
Momozawa, Y;Takeuchi, Y;Mori, Y
据报道，多巴胺D4受体（DRD 4）基因的可变数量串联重复（VNTR）多态性与人类寻求新奇的人格特征相关。在马属中，该区域包括一个18 bp的重复单元，并且重复数量存在种间和种内差异。由于马是独特的牲畜物种，他们的气质被认为是重要的，我们调查了这一地区的马的气质在纯种马可能发挥的作用。我们同时确定了这个多态性区域的序列，并进行了问卷调查，以马管理员的问题，约20个不同的特点，他们的马的气质。虽然有136匹纯种马的重复序列的数量没有差异，两个单核苷酸多态性（SNP），其中之一可能会导致氨基酸的变化（A-G取代），存在。通过分析这些SNPs与气质得分之间的关联，揭示了两个气质性状（Curvilance和Vigilance）与A-G替换之间的显著关联。在A-G置换时，无A等位基因的马比有A等位基因的马具有显著更高的Curcillance和更低的Vigilance评分。此外，在根据引入农场的时间划分的两个亚组中观察到气质评分和A-G置换的每个基因型之间的相似关联。这些结果表明，马DRD 4基因VNTR区域的SNP可能与马的气质个体差异有关。
The variable number of tandem repeats (VNTR) polymorphism of the dopamine D4 receptor (DRD4) gene has been reported to be associated with the personality trait of novelty-seeking in humans. In the genus Equus, this region includes an 18-bp repeat unit and there are inter- and intraspecies differences in the number of repetitions. Because horses are unique among livestock species in that their temperament is considered important, we investigated the possible role of this region on equine temperament in thoroughbred horses. We simultaneously determined the sequences of this polymorphic region and administered a questionnaire survey to horse caretakers with questions about 20 different traits of their horses' temperament. Although there was no difference in the number of repeats among the 136 thoroughbred horses studied, two single nucleotide polymorphisms (SNPs), one of which might cause an amino acid change (A-G substitution), existed. By analyzing the association between these SNPs and temperament scores, a significant association was revealed between two temperament traits (Curiosity and Vigilance) and the A-G substitution. Horses without the A allele had significantly higher Curiosity and lower Vigilance scores than those with the A allele at the A-G substitution. In addition, similar associations between both temperament scores and each genotype of the A-G substitution were observed in two subgroups divided according to the time of their introduction to the farm. These results suggested that the SNP in the VNTR region of the equine DRD4 gene might be related to individual differences in equine temperament.