Assessing bioenergetic function in response to oxidative stress by metabolic profiling.
Assessing bioenergetic function in response to oxidative stress by metabolic profiling.
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DOI：
10.1016/j.freeradbiomed.2011.08.005
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发表时间：
2011-11-01
影响因子：
7.4
通讯作者：
Darley-Usmar VM
中科院分区：
文献类型：
--
作者：
Dranka BP;Benavides GA;Diers AR;Giordano S;Zelickson BR;Reily C;Zou L;Chatham JC;Hill BG;Zhang J;Landar A;Darley-Usmar VM
现在清楚的是，线粒体是包括心血管疾病、糖尿病、神经退行性变和癌症在内的广泛病理中氧化应激的重要靶点。评估活性物种对分离线粒体的影响的方法已经建立得很好，但由于需要大量材料来准备用于极谱测量的完整线粒体，因此受到限制。有了高分辨率极谱和荧光技术来测量溶液中的氧浓度，就可以测量完整细胞的线粒体功能。近年来，细胞外通量法的发展，可以同时监测多个样品井中贴壁细胞培养物中氧浓度和pH的变化，大大提高了测量生物能量功能响应氧化应激的能力。在这里，我们使用来自肾脏、心血管、神经和肿瘤模型系统的代表性细胞类型来详细描述这些方法，同时说明三种方案的应用来分析细胞对氧化应激的生物能量响应。
It is now clear that mitochondria are an important target for oxidative stress in a broad range of pathologies including cardiovascular disease, diabetes, neurodegeneration, and cancer. Methods for assessing the impact of reactive species on isolated mitochondria are well established but constrained by the need for large amounts of material to prepare intact mitochondria for polarographic measurements. With the availability of high resolution polarography and fluorescence techniques for the measurement of oxygen concentration in solution, measurements of mitochondrial function in intact cells can be made. Recently, the development of extracellular flux methods to monitor changes in oxygen concentration and pH in cultures of adherent cells in multiple sample wells simultaneously has greatly enhanced the ability to meaure bioenergetic function in response to oxidative stress. Here we describe these methods in detail using representative cell types from the renal, cardiovascular, nervous, and tumorigenic model systems while illustrating the application of three protocols to analyze the bioenergetic response of cells to oxidative stress.
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影响因子：
7.4
作者：
Gerencser, Akos A.;Neilson, Andy;Choi, Sung W.;Edman, Ursula;Yadava, Nagendra;Oh, Richard J.;Ferrick, David A.;Nicholls, David G.;Brand, Martin D.
通讯作者：
Brand, Martin D.
影响因子：
7.4
作者：
Dranka, Brian P.;Hill, Bradford G.;Darley-Usmar, Victor M.
通讯作者：
Darley-Usmar, Victor M.
影响因子：
2.8
作者：
Chuang, CK;Wang, TJ;Lin, SP
通讯作者：
Lin, SP
DOI：
10.1152/ajpheart.00210.2011
发表时间：
2011-09-01
影响因子：
4.8
作者：
Diers, Anne R.;Broniowska, Katarzyna A.;Hogg, Neil
通讯作者：
Hogg, Neil
影响因子：
4.8
作者：
Hwang, J;Saha, A;Jo, H
通讯作者：
Jo, H