Accumulation of very-long-chain fatty acids in membrane glycerolipids is associated with dramatic alterations in plant morphology
Accumulation of very-long-chain fatty acids in membrane glycerolipids is associated with dramatic alterations in plant morphology
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DOI：
10.1105/tpc.10.11.1889
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发表时间：
1998-11-01
期刊：
影响因子：
11.6
通讯作者：
Kunst, L
中科院分区：
文献类型：
--
作者：
Millar, AA;Wrischer, M;Kunst, L
在花椰菜花叶病毒35S启动子的控制下，高表达拟南芥脂肪酸ELONGATION1基因的转基因植株在植株中积累了超长链脂肪酸(VLCFA)。在一些转化子中，C20和C22 VLCFA占总脂肪酸的30%，积累的代价是C16和C18脂肪酸。这些C20和C22脂肪酸被结合到所有主要的膜甘油脂类中。VLCFA含量高的植株表现出明显的形态变化，包括花枝不能伸长，角果的空间格局发生改变，花型发生变化，花青素大量积累。此外，这些植物还表现出叶绿体膜结构的独特变化。我们讨论了VLCFA在确定膜的形状/曲率中的可能作用，这反过来可能影响细胞的形状，并最终影响整个植物的形状。
Transgenic Arabidopsis plants overexpressing the Arabidopsis FATTY ACID ELONGATION1 gene under the control of the 35S promoter from cauliflower mosaic virus accumulated very-long-chain fatty acids (VLCFAs) throughout the plant. In some transformants, C20 and C22 VLCFAs accounted for >30% of the total fatty acids, accumulating at the expense of C16 and C18 fatty acids. These C20 and C22 fatty acids were incorporated into all of the major membrane glycerolipid classes. Plants with a high VLCFA content displayed a dramatically altered morphology, which included the failure of flowering shoots to elongate, a modified spatial pattern of siliques, an altered floral phenotype, and a large accumulation of anthocyanins. In addition, these plants also exhibited a unique alteration of the chloroplast membrane structure. We discuss a possible role for VLCFAs in establishing the shape/curvature of the membranes, which in turn may affect the shape of the cell and ultimately that of the whole plant.