ANCESTRY OF THE 4-CHLOROBENZOATE DEHALOGENASE - ANALYSIS OF AMINO-ACID-SEQUENCE IDENTITIES AMONG FAMILIES OF ACYL-ADENYL LIGASES, ENOYL-COA HYDRATASES ISOMERASES, AND ACYL-COA THIOESTERASES
ANCESTRY OF THE 4-CHLOROBENZOATE DEHALOGENASE - ANALYSIS OF AMINO-ACID-SEQUENCE IDENTITIES AMONG FAMILIES OF ACYL-ADENYL LIGASES, ENOYL-COA HYDRATASES ISOMERASES, AND ACYL-COA THIOESTERASES
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DOI：
10.1021/bi00139a024
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发表时间：
1992-06-23
期刊：
影响因子：
2.9
通讯作者：
DUNAWAYMARIANO, D
中科院分区：
文献类型：
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作者：
BABBITT, PC;KENYON, GL;DUNAWAYMARIANO, D
我们推导了假单胞菌CBS-3的4-氯苯甲酸（4-CBA）脱卤酶三个组分的编码基因的核苷酸序列，并通过同源性分析研究了这些蛋白质的来源。开放阅读框1（ORF 1）编码30-kDa的4-CBA-辅酶A脱卤酶，其与在脂肪酸β-氧化中起作用的烯酰辅酶A水合酶相关。ORF 2编码一个57-kDa的蛋白质，其通过酰基腺苷酸化/硫代酯化作用激活4-CBA。这种4-CBA：辅酶A连接酶与一大组蛋白质具有显著的序列相似性，其中许多蛋白质在β-氧化途径或铁载体和抗生素合成途径中催化类似的化学反应。这些蛋白质共同具有一段短的序列，（T，S）（S，G）G（T，S）（T，E）G（L，X）PK（G，-），其特别高度保守，并且其可以代表一类重要的新的“特征”序列。我们无法找到任何蛋白质同源序列的16 kDa的4-羟基苯甲酸辅酶A硫酯酶编码的ORF 3。对发现与4-CBA：辅酶A连接酶和4-CBA-辅酶A脱卤酶结构相关的蛋白质的化学和功能的分析支持了它们从β-氧化途径进化的提议。
We have deduced the nucleotide sequence of the genes encoding the three components of 4-chlorobenzoate (4-CBA) dehalogenase from Pseudomonas sp. CBS-3 and examined the origin of these proteins by homology analysis. Open reading frame 1 (ORF1) encodes a 30-kDa 4-CBA-coenzyme A dehalogenase related to enoyl-coenzyme A hydratases functioning in fatty acid beta-oxidation. ORF2 encodes a 57-kDa protein which activates 4-CBA by acyl adenylation/thioesterification. This 4-CBA:coenzyme A ligase shares significant sequence similarity with a large group of proteins, many of which catalyze similar chemistry in beta-oxidation pathways or in siderophore and antibiotic synthetic pathways. These proteins have in common a short stretch of sequence, (T,S)(S,G)G(T,S)(T,E)G(L,X)PK(G,-), which is particularly highly conserved and which may represent an important new class of "signature" sequence. We were unable to find any proteins homologous in sequence to the 16-kDa 4-hydroxybenzoate-coenzyme A thioesterase encoded by ORF3. Analysis of the chemistry and function of the proteins found to be structurally related to the 4-CBA:coenzyme A ligase and the 4-CBA-coenzyme A dehalogenase supports the proposal that they evolved from a beta-oxidation pathway.