The decreased permittivity of zebrafish embryos culture medium by magnetic fields did not affect early development of zebrafish embryos
The decreased permittivity of zebrafish embryos culture medium by magnetic fields did not affect early development of zebrafish embryos
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磁场降低斑马鱼胚胎培养基的介电常数并不影响斑马鱼胚胎的早期发育
DOI：
10.1016/j.ecoenv.2020.110350
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发表时间：
2020
影响因子：
6.8
通讯作者：
Chen Guangdi
中科院分区：
文献类型：
--
作者：
Su Liling;Zhu Longtao;Liu Zhenchao;Lou Jianyao;Han Bing;Lin Chen;Li Dongyu;Qian Jun;Zhao Xinyuan;Chen Guangdi
流行病学研究表明，暴露于环境极低频磁场(ELF-MF)与健康效应之间存在关联，但ELF-MF诱导的生物效应的机制尚不清楚。我们推测ELF-MF可能通过其对周围环境的影响来调节组织或细胞的功能。为了验证这一假设，我们研究了50赫兹高频磁场对斑马鱼胚胎培养液相对介电常数的影响以及磁场暴露对斑马鱼胚胎发育的影响。用相敏表面等离子体共振(SPR)系统评估中至50 GHz中频辐射的响应。结果表明，MF处理降低了斑马鱼胚胎培养液的相对介电常数，且呈剂量和时间依赖关系。有趣的是，MF暴露引起的介电常数下降逐渐恢复，并在移除暴露后接近基础水平。然而，在斑马鱼胚胎发育过程中，MF预先暴露的培养液不会引发胚胎的不良后果，包括死亡、畸形、孵化和心率。此外，在受精后48h和72h，暴露于MF的培养液不能诱导斑马鱼胚胎的凋亡。我们的数据表明，暴露于MF后，斑马鱼胚胎培养液的相对介电常数降低，但这种降低并未导致斑马鱼胚胎的异常发育。
Epidemiological studies have shown associations between exposure to environmental extremely low frequency magnetic fields (ELF-MF) and health effects, but the mechanisms of ELF-MF induced biological effects remain unclear. We hypothesized that ELF-MF may regulate functions of tissues or cells via its effects on surrounding environment, e.g., culture medium. To test this hypothesis, we investigated the effects of 50 Hz MF on the relative permittivity of zebrafish embryos culture medium as well as of MF-exposed medium on zebrafish embryos development. The responses of medium to 50 Hz MF exposure were evaluated by a phase-sensitive surface plasmon resonance (SPR) system. The results demonstrated that MF treatment decreased relative permittivity of zebrafish embryos medium in a dose and time-dependent way. Interestingly, the decreased permittivity induced by MF exposure gradually recovered and approached to the base level when the exposure was removed off. However, MF-exposed medium did not trigger adverse consequences of embryos during zebrafish embryonic development, including mortality, malformation, hatching and heart rate when the MF pre-exposed medium was subjected to one cell-stage embryos. Moreover, the MF-exposed medium did not induce apoptosis of zebrafish embryos at 48 and 72 h post fertilization. Our data demonstrated that the relative permittivity of zebrafish embryos medium was decreased by MF exposure, whereas this decrease failed to result in abnormal development of zebrafish embryos.