SARS-CoV-2 detection by direct rRT-PCR without RNA extraction.
SARS-CoV-2 detection by direct rRT-PCR without RNA extraction.
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DOI：
10.1016/j.jcv.2020.104423
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发表时间：
2020-07-01
期刊：
影响因子：
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通讯作者：
Danylo, Alexis
中科院分区：
文献类型：
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作者：
Merindol, Natacha;Pepin, Genevieve;Danylo, Alexis
快速可靠地筛查SARS-CoV-2对于评估病毒传播和限制我们所面临的大流行至关重要。本研究比较了SeeGene AllplexTM 2019-nCoV rRT-PCR与RealStar SARS-CoV-2 rRT-PCR试剂盒（Altona Diagnostics）的直接rRT-PCR方法（不提取RNA）。此外，我们评估了棉签储存介质组成对PCR效率的影响。我们发现SeeGene和Altona的检测方法提供了相似的效率。重要的是，我们提供的证据表明，当样品储存在UTM介质或分子水中时，RNA提取可以成功绕过，而当样品储存在盐水溶液和Hanks介质中时则不能。
Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). Furthermore, we assessed the impact of swab storage media composition on PCR efficiency. We show that SeeGene and Altona's assays provide similar efficiency. Importantly, we provide evidence that RNA extraction can be successfully bypassed when samples are stored in UTM medium or in molecular water but not when samples are stored in saline solution and in Hanks medium.