Secretion of the toxin ExoU is a marker for highly virulent Pseudomonas aeruginosa isolates obtained from patients with hospital-acquired pneumonia
Secretion of the toxin ExoU is a marker for highly virulent Pseudomonas aeruginosa isolates obtained from patients with hospital-acquired pneumonia
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DOI：
10.1086/379372
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发表时间：
2003-12-01
影响因子：
6.4
通讯作者：
Hauser, AR
中科院分区：
文献类型：
--
作者：
Schulert, GS;Feltman, H;Hauser, AR
总体而言，铜绿假单胞菌引起的医院获得性肺炎（HAP）与高归因死亡率相关。虽然铜绿假单胞菌的内在毒力无疑有助于这种现象，但尚不清楚是否所有菌株都具有这种特性，或者是否只有菌株亚群能够引起这种严重的疾病。在这项研究中，35个铜绿假单胞菌分离株获得的HAP患者使用细胞溶解细胞死亡试验，细胞凋亡试验，和小鼠肺炎模型的毒力。在这些试验中，单个分离株的毒力彼此之间存在显著差异。毒力增加与ExoU的分泌有关，ExoU是一种由铜绿假单胞菌III型分泌系统转运的毒素。分泌ExoS或ExoY，2种由该系统转运的其他蛋白质，与毒力增加并不一致。总之，这些发现表明ExoU的分泌是铜绿假单胞菌高毒力菌株的标志物。
Overall, hospital-acquired pneumonia (HAP) caused by Pseudomonas aeruginosa is associated with high attributable mortality. Although the intrinsic virulence of P. aeruginosa undoubtedly contributes to this phenomenon, it is unclear whether all strains share this property or whether only a subpopulation of strains are capable of causing such severe disease. In this study, the virulence of 35 P. aeruginosa isolates obtained from patients with HAP by use of a cytolytic cell-death assay, an apoptosis assay, and a mouse model of pneumonia. The virulence of individual isolates differed significantly from one to another in each of these assays. Increased virulence was associated with the secretion of ExoU, a toxin transported by the P. aeruginosa type III secretion system. Secretion of ExoS or ExoY, 2 other proteins transported by this system, was not consistently associated with increased virulence. Together, these findings suggest that secretion of ExoU is a marker for highly virulent strains of P. aeruginosa.