Prevalence and molecular characterization of cystic hydatidosis in livestock slaughtered in southern Mozambique.
Prevalence and molecular characterization of cystic hydatidosis in livestock slaughtered in southern Mozambique.
复制标题
莫桑比克南部屠宰牲畜囊性包虫病的患病率和分子特征。
DOI：
10.1007/s12639-021-01434-6
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Mukaratirwa,S
中科院分区：
文献类型：
--
作者：
Miambo,RD;Afonso,SMS;Noormahomed,EV;Malatji,MP;Mukaratirwa,S
包虫病是一种由棘球绦虫属基因分型引起的人畜共患寄生虫病。这种疾病给牲畜造成经济损失，对资源贫乏的人造成公共卫生负担，主要是在发展中国家。这项研究的目的是确定在莫桑比克南部省份选定的屠宰场屠宰的牛、山羊和猪中导致包虫病的基因类型的流行率和身份。取肝、肺包囊，通过囊膜观察和光镜下观察原头节形态，确认包虫。从当地屠宰场和猪场采集了817头牛和68头猪的32个包虫包囊和2个包虫包囊。从每个包囊的原头节和囊膜中提取DNA，根据COX1和NAD1基因的线粒体亚单位1进行扩增。牛、猪包虫总患病率分别为3.9%、2.9%，未发现山羊包囊。经BLAST分析，所有采集自牛和猪的包囊均为细粒棘球绦虫(G5)，最低同源性为99%。我们的结果证实了E。建议进行进一步研究，以确定有利于这种人畜共患寄生虫在该地区资源贫乏的畜牧业社区传播的风险因素。
Hydatid disease is a parasitic zoonosis caused by genotypes of the genusEchinococcus. This disease inflicts economic loses in livestock and cause public health burden in resource poor mostly in developing countries. The aim of this study was to determine the prevalence and identity of the genotypes responsible for hydatid cysts in cattle, goats and pigs slaughtered at selected abattoirs of southern provinces of Mozambique. Cysts were collected from liver and lungs and hydatid confirmation was made by cystic membrane observation and visualization of protoscoleces by light microscope. Thirty-two hydatid cysts from 817 cattle and two from 68 pigs were collected from local slaughterhouses and slabs. DNA was extracted from protoscoleces of each cyst together with the cystic membrane and amplified based on the mitochondrial subunit 1 of thecox1andnad1gene. The overall prevalence of hydatid cysts was 3.9% in cattle, 2.9% in pigs and none of the goats were found with cysts. All cysts collected from cattle and pigs were identified asEchinococcus ortleppi(G5) with a minimum homology of 99% on BLAST analysis. Our results confirm the presence ofE. ortleppiin cattle and pigs in southern Mozambique at a low prevalence and further studies are recommended to determine the risk factors favoring the transmission of this zoonotic parasite in the resource-poor livestock farming communities of this region.