Interaction of calmodulin with skeletal muscle myosin light chain kinase.
Interaction of calmodulin with skeletal muscle myosin light chain kinase.
复制标题
钙调蛋白与骨骼肌肌球蛋白轻链激酶的相互作用。
DOI：
10.1021/bi00525a006
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Potter,JD
中科院分区：
文献类型：
--
作者：
Crouch,TH;Holroyde,MJ;Collins,JH;Solaro,RJ;Potter,JD
1来自辛辛那提大学医学院，辛辛那提，俄亥俄州45267，药理学和细胞生物物理学系（THC、JHC、RJS和JDP）收缩蛋白科。1981年4月20日收到。这项工作得到了美国国立卫生研究院赠款（HL 22619-3A，B，E）HL 22231和AM-20875，博士后研究培训赠款HL 07382，肌营养不良协会和美国心脏协会（78-1167）的支持。THC是肌肉营养不良协会的成员。JHC和RJS是研究职业发展奖的持有者。链浓度证实了这一观察结果。酶-钙调素复合物的激活的钙依赖性的特征在于希尔系数为2.5，半激活发生在6.6 X1 ct 7 M Ca 2+。氨基酸组成显示脯氨酸的高百分比（9.1%），这可能是大的表观Stokes半径和其他骨骼肌蛋白没有明显的相似之处。与火鸡砂囊的氨基酸组成比较显示出一些相似之处。Ca 2+依赖性肌球蛋白轻链激酶（MLCK）。1在完整的骨骼肌中，这发生在收缩期间（Bárány等人，1979），并可能在肌肉中的峰值抽搐张力的强直后增强中起作用（Manning & Stull，1979）。
1 From the Section of Contractile Proteins, Department of Pharma-cology and Cell Biophysics (THC, JHC, RJS, and JDP), and the Department of Physiology (MJH and RJS), University of Cincinnati College of Medicine, Cincinnati, Ohio 45267. Received April 20, 1981. This work was supported by National Institutes of Health Grants (HL 22619-3A, B, E) HL 22231 and AM-20875, Postdoctoral Research Training Grant HL 07382, the Muscular Dystrophy Association, and the American Heart Association (78-1167). THC is a fellow of the Muscular Dystrophy Association. JHC and RJS are holders of Research Career Development Awards. chain concentrations confirms this observation. The calcium dependence of activation of the enzyme-calmodulin complex is characterized by a Hill coefficient of 2.5, with half-activation occurring at 6.6 X1ct7 M Ca2+. The amino acid composition shows a high percentage (9.1%) of proline, which may account for the large apparentStokes radius and no clear resemblance to other skeletal muscle proteins. A comparison of the amino acid composition with that from turkey gizzard shows some resemblance. by a Ca2+-dependent myosin light chain kinase (MLCK). 1 In intact skeletal muscle, this occurs during contraction (Bárány et al., 1979) and may play a role in the posttetanic potentiation of peak twitch tension in the muscle (Manning & Stull, 1979).