Zm401, a short-open reading-frame mRNA or noncoding RNA, is essential for tapetum and microspore development and can regulate the floret formation in maize
Zm401, a short-open reading-frame mRNA or noncoding RNA, is essential for tapetum and microspore development and can regulate the floret formation in maize
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DOI：
10.1002/jcb.21807
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发表时间：
2008-09-01
影响因子：
4
通讯作者：
Ao, Guangming
中科院分区：
文献类型：
--
作者：
Ma, Jinxia;Yan, Bingxue;Ao, Guangming
在开花植物中，花粉的形成取决于花药中两种细胞类型的分化和相互作用：称为小孢子母细胞的生殖细胞和形成绒毡层的体细胞。在此之前，我们从玉米成熟花粉cDNA文库中克隆了花粉特异表达基因zm 401。zm 401基因在玉米中的部分cDNA表达和在烟草中的异位表达表明其在花药发育中起作用。在这里，我们介绍了玉米花粉特异性基因的表达和功能特征。Zm 401主要在花药（绒毡层细胞以及小孢子）中以发育调节的方式表达。也就是说，它在小花形成期前表达，直到成熟花粉浓度增加。Zm 401的敲低显著影响ZmMADS 2的表达。MZm3-3和ZmC 5基因，导致小孢子和绒毡层发育异常，最终导致雄性不育。Zm 401在单子叶植物中具有高度保守的序列和进化上保守稳定的RNA二级结构。这些数据表明zm 401可能是花药发育中的关键生长调节因子之一，并且作为短开放阅读框mRNA（sORF mRNA）和/或非编码RNA（mRNA）发挥功能。
In flowering plants, pollen formation depends oil the differentiation and interaction of two cell types in the anther: the reproductive cells, called microsporocytes, and somatic cells that form the tapetum. Previously, we cloned a pollen specific gene, zm401, front a cDNA library generated from the mature pollen of Zea mays. Expression of partial cDNA of zm401 in maize and ectopic expression of zm401 in tobacco suggested it play play a role in anther development. Here we present the expression and functional characterization of this pollen specific gene ill maize. Zm401 is expressed primarily in the anthers (tapetal cells as well as microspores) in a developmentally regulated manner. That is, it is expressed front floret forming stage, increasing in concentration up to mature pollen. Knockdown of zm401 significantly affected the expression of ZmMADS2. MZm3-3. and ZmC5, critical genes for pollen development; led to aberrant development of the microspore and tapetum, and finally male-sterility. Zm401 possesses highly Conserved sequences and evolutionary conserved stable RNA secondary structure in monocotyledon. These data show that zm401 could be one of the key growth regulators in anther development, and functions as a short-open reading-frame mRNA (sORF mRNA) and/or noncoding RNA (mRNA).