Danshensu is the major marker for the antioxidant and vasorelaxation effects of Danshen (Salvia miltiorrhiza) water-extracts produced by different heat water-extractions
Danshensu is the major marker for the antioxidant and vasorelaxation effects of Danshen (Salvia miltiorrhiza) water-extracts produced by different heat water-extractions
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DOI：
10.1016/j.phymed.2012.08.011
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发表时间：
2012-11-15
期刊：
影响因子：
7.9
通讯作者：
Yeung, John H. K.
中科院分区：
文献类型：
--
作者：
Zhou, Xuelin;Chan, Shun Wan;Yeung, John H. K.
丹参是一种广泛使用的中草药，富含酚酸，其一些主要成分对热敏感，在加热提取过程中可能会降解为其他酚酸。丹参水提取物的化学成分可能会随着不同温度下的热水提取而变化，从而影响提取物的成分和生物活性。本研究对不同温度下热回流水提取和微波辅助水提取（MAE-W）获得的六种丹参水提取物的成分和药理作用进行了测试。其中，第三轮MAE-W（100℃）提取物的酚酸和丹参酮含量最高，在2,2-二苯基-1-(2,4,6-三硝基苯基)肼（DPPH）测定和铁还原/抗氧化电位（FRAP）测定中抗氧化活性最强。该提取物还对 2,2'-偶氮双-2-脒基丙烷 (AAPH) 诱导的人红细胞溶血、过氧化氢诱导的大鼠心脏 H9c2 细胞凋亡具有最强的抑制作用，并对大鼠基底动脉具有最强的松弛作用。丹参水提取物的抗氧化作用与其放松作用线性相关（r = 0.895-0.977）。通过多元线性回归分析，发现丹参素是丹参水提取物抗氧化和舒血管作用中最显着的标志物，而丹参酮IIA是过氧化氢诱导的大鼠心脏H9c2细胞凋亡的标志物。因此，丹参素是丹参水提取物抗氧化和血管舒张质量控制的有用标记，而丹参酮 IIA 则是不同提取物抗凋亡潜力的有用标记。 (C) 2012 年爱思唯尔有限公司。版权所有。
Some of the major components of Danshen (Salvia miltiorrhiza), a widely used Chinese herbal medicine rich in phenolic acids, are thermosensitive and may degrade to other phenolic acids during extractions with heating. The chemical profiles of Danshen water-extract may vary with different heat water extraction at different temperatures, affecting the composition and bioactivity of the extracts. In this study, six water-extracts of Danshen obtained from heat reflux water extraction and microwave-assisted extraction with water (MAE-W) at different temperatures were tested for their composition and pharmacological effects. Among these extracts, the third-round MAE-W (100 degrees C) extract had the highest phenolic acids and tanshinones contents, with the strongest antioxidant activity in 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay and ferric reducing/antioxidant potential (FRAP) assay. This extract also showed the strongest inhibitory effects on 2,2'-azobis-2-amidinopropane (AAPH)-induced hemolysis in human red blood cells, hydrogen peroxide-induced apoptosis in rat heart H9c2 cells and the highest relaxation effects on rat basilar artery. The antioxidant effects of Danshen water-extracts linearly correlated to their relaxation effects (r = 0.895-0.977). Through multiple linear regression analysis, danshensu was found to be the most significant marker in the antioxidant and vasodilation effects of Danshen water-extract, while tanshinone IIA as the marker on hydrogen peroxide-induced apoptosis in rat heart H9c2 cells. Danshensu is, therefore, a useful marker for the quality control of Danshen water-extracts in antioxidant and vasodilation, while tanshinone IIA for anti-apoptotic potential of different extracts. (C) 2012 Elsevier GmbH. All rights reserved.