Possible role of trichothecene mycotoxins in virulence of Fusarium graminearum on maize
Possible role of trichothecene mycotoxins in virulence of Fusarium graminearum on maize
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DOI：
10.1094/pdis.1999.83.10.954
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发表时间：
1999-10-01
期刊：
影响因子：
4.5
通讯作者：
Hohn, TM
中科院分区：
文献类型：
--
作者：
Harris, LJ;Desjardins, AE;Hohn, TM
1996年，在两个地点--安大略的渥太华和伊利诺斯州的皮奥里亚--的田间试验中，测试了产生单端孢霉烯和不产生单端孢霉烯的禾谷镰刀菌菌株引起赤霉病的能力。玉米穗接种野生型或转基因F。禾谷镰刀菌菌株，其中，通过特异性破坏麦二烯合酶基因使麦二烯生物合成途径失效，以及衍生的回复突变菌株，其中，通过重组恢复了麦二烯的生产。在两个位置采用丝通道接种法。此外，在安大略地区使用了籽粒穿刺接种法。收获的玉米穗进行了分析的视觉疾病的严重程度，粮食产量，脱氧雪腐镰刀菌烯醇（DON）的浓度，和真菌生物量的定量聚合酶链反应（PCR）和/或麦角甾醇定量。有一个显着的相关性（r = 0.86）从两种不同的方法定量真菌生物量获得的数据之间。不产生二孢霉素的菌株仍然是致病性的，但似乎比产生二孢霉素的祖细胞和回复突变株对玉米的毒力较低，大多数参数测定。这表明，该菌可能作为毒力因子，以促进F。graminearum在玉米上。
Trichothecene-producing and -nonproducing Fusarium graminearum strains were tested for their ability to cause Gibberella ear rot in field trials at two locations-Ottawa, Ontario, and Peoria, Illinois-in 1996. Maize ears were inoculated with wild-type or transgenic F. graminearum strains in which the trichothecene biosynthetic pathway had been disabled by the specific disruption of the trichodiene synthase gene and with a derivative revertant strain in which trichothecene production had been restored through recombination. A silk channel inoculation method was employed at both locations. In addition, a kernel puncture inoculation method was used at the Ontario location. Harvested maize ears were analyzed for visual disease severity, grain yield, deoxynivalenol (DON) concentration, and fungal biomass by quantitative polymerase chain reaction (PCR) and/or ergosterol quantitation. There was a significant correlation (r = 0.86) between data obtained from the two different methods of quantifying fungal biomass. The trichothecene-nonproducing strains were still pathogenic but appeared less virulent on maize than the trichothecene-producing progenitor and revertant strains, as assayed by most parameters. This suggests that the trichothecenes may act as virulence factors to enhance the spread of F. graminearum on maize.